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U7128

Sigma-Aldrich

Uricase from Arthrobacter globiformis

lyophilized powder, 15-30 units/mg protein (biuret)

Synonyme(s) :

Urate oxidase, Urate:O2 oxidoreductase

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About This Item

Numéro CAS:
9002-12-4
Numéro de classification (Commission des enzymes):
1.7.3.3 (BRENDA, IUBMB)
Numéro CE :
232-655-5
Numéro MDL:
MFCD00082126
Code UNSPSC :
12352204
ID de substance PubChem :
24900663
Nomenclature NACRES :
NA.54

Forme

lyophilized powder

Activité spécifique

15-30 units/mg protein (biuret)

Poids mol.

tetramer ~32 kDa

Température de stockage

−20°C

InChI

1S/C18H23N4O14P.H3N/c23-5-7-14(13(28)16(34-7)22-4-2-10(25)20-18(22)30)36-37(31,32)33-6-8-11(26)12(27)15(35-8)21-3-1-9(24)19-17(21)29;/h1-4,7-8,11-16,23,26-28H,5-6H2,(H,31,32)(H,19,24,29)(H,20,25,30);1H3

Clé InChI

VPXDHZMLJOJKOX-UHFFFAOYSA-N

Description générale

Research area: Cell Signalling. Uricase is made of four identical subunits and is a homotetrameric enzyme. It is found as a functional uricase in several organisms including, bacteria, archaea, and eukaryotes. Nonetheless, primates and humans lack a functional uricase enzyme.

Application

Uricase from Arthrobacter globiformis has been used for coating the electrodes in - biosensor fabrication for the detection of uric acid in urine samples. It has also been used for analysing the effect of Zn, Cu, Mg, and Mn ions on uricase.

Actions biochimiques/physiologiques

Arthrobacter globiformis is a major producer of uricase or urate oxidase in poultry manure and litter. Uricase hydrolyzes uric acid and causes ammonia volatilization in poultry manure. In the majority of mammals, uricase converts uric acid to allantoin, a soluble excretion product. Humans lack uricase due to its loss during the evolution of hominids. This increases the risk of humans towards gout, kidney stones, hypertension, and diabetes due to increased circulating levels of uric acid.Uricase is an important enzyme that plays a role in the purine metabolic pathway.
Molecular weight: 4 × ~32 KDa (tetramer)
Isoelectric point: is 4.64
KM value (uric acid as substrate): ~ 0.01 mM
The optimum pH for activity: 8.5-9.5
The optimum pH for stability: 6.0-9.5
Zn and Cu were shown to inhibit the activity of uricase.

Définition de l'unité

One unit will convert 1.0 μmole of uric acid to allantoin per min at pH 9.0 at 25 °C.

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H334

Conseils de prudence

P261 - P284 - P501

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

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Consulter la Bibliothèque de documents

Uric acid: a danger signal from the RNA world that may have a role in the epidemic of obesity, metabolic syndrome, and cardiorenal disease: evolutionary considerations.
Seminars in Nephrology, 31(5), 394-399 (2011)
W K Kim et al.
Poultry science, 82(2), 223-231 (2003-03-07)
Inhibition of microbial uricase in poultry manure is critical to reduce NH3 volatilization, because hydrolysis of uric acid by microbial uricase is the first step in the production of NH3 gas in poultry manure. Three experiments were conducted to evaluate
Development of a sandwich format, amperometric screen-printed uric acid biosensor for urine analysis.
Kanyong P, et al.
Analytical biochemistry, 428(1), 39-43 (2012)
Uric acid and evolution.
Bonifacio AL and Jesus MV
Rheumatology (Basel), 49(11), 2010-2015 (2010)
Effect of minerals on activity of microbial uricase to reduce ammonia volatilization in poultry manure.
Kim WK and Patterson PH
Poultry Science, 82(2), 223-231 (2003)
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