T7254

N_α-Tosyl-L-lysine chloromethyl ketone hydrochloride

powder, ≥96% (TLC)

• Synonyme(s) : (3S)-1-Chloro-3-tosylamido-7-amino-2-heptanone hydrochloride, (3S)-7-Amino-1-chloro-3-tosylamino-2-heptanone hydrochloride, TLCK, Tosyl-L-lysyl-chloromethane hydrochloride
• Formule empirique (notation de Hill): C₁₄H₂₁ClN₂O₃S · HCl
• Numéro CAS: 4272-74-6
• Poids moléculaire : 369.31
• Numéro Beilstein : 7106867
• Numéro CE : 224-266-4
• Numéro MDL: MFCD00065395
• Code UNSPSC : 12352202
• ID de substance PubChem : 24278752
• Nomenclature NACRES : NA.77

Propriétés

• product name: N_α-Tosyl-L-lysine chloromethyl ketone hydrochloride, ≥96% (TLC), powder
• Source biologique: synthetic (organic)
• Niveau de qualité: 200
• Pureté: ≥96% (TLC)
• Forme: powder
• Puissance: 10-100 μM effective concentration
• Solubilité: H₂O: 50 mg/mL
• Température de stockage: −20°C
• Chaîne SMILES: Cl[H].Cc1ccc(cc1)S(=O)(=O)N[C@@H](CCCCN)C(=O)CCl
• InChI: 1S/C14H21ClN2O3S.ClH/c1-11-5-7-12(8-6-11)21(19,20)17-13(14(18)10-15)4-2-3-9-16;/h5-8,13,17H,2-4,9-10,16H2,1H3;1H/t13-;/m0./s1
• Clé InChI: YFCUZWYIPBUQBD-ZOWNYOTGSA-N
• Informations sur le gène: human ... NFKB1(4790) , NFKB2(4791)

Description

Application

Nα-Tosyl-L-lysine chloromethyl ketone hydrochloride has been used:

• in chymotrypsin purification to prevent binding of trypsins to the affinity support by inhibiting them in the crude extract
• as a protease inhibitor in tissue and cell homogenization
• as a trypsin inhibitor to determine the specific protease activity levels

Actions biochimiques/physiologiques

Nα-Tosyl-L-lysine chloromethyl ketone hydrochloride (TLCK) blocks the lipopolysaccharide (LPS)- or cytokine-induced activation of nuclear factor κB (NF-κB), which, in turn, blocks the induction of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) transcription. Blocks activation of pp70s6k by all mitogens. Blocks apoptosis in cell lines by inhibiting the processing of caspases in some cell lines and to some stimuli.

Conditionnement

Bottomless glass bottle. Contents are inside inserted fused cone.

Attention

Stable at least two years when stored desiccated at −20°C.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: dust mask type N95 (US), Eyeshields, Gloves