T6066

Trizma^® base

BioPerformance Certified, meets EP, USP testing specifications, suitable for cell culture, ≥99.9% (titration)

• Synonyme(s) : 2-Amino-2-(hydroxyméthyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxyméthyl)aminométhane, Trométamol
• Formule linéaire : NH₂C(CH₂OH)₃
• Numéro CAS: 77-86-1
• Poids moléculaire : 121.14
• Numéro Beilstein : 741883
• Numéro CE : 201-064-4
• Numéro MDL: MFCD00004679
• Code UNSPSC : 12352104
• ID de substance PubChem : 24900345
• Nomenclature NACRES : NA.25

Propriétés

• Qualité: BioPerformance Certified
• Niveau de qualité: 400
• Agence: USP/NF; meets EP testing specifications; meets USP testing specifications
• Description: aminopeptidase substrate
• Pureté: ≥99.9% (titration)
• Forme: crystalline powder
• Conditions de stockage: dry at room temperature
• Technique(s): DNA extraction: suitable; cell culture | mammalian: suitable; protein purification: suitable
• Impuretés: DNase, RNase, protease, none detected; Endotoxin and Total Count Aerobic, Yeast, plus Mold, tested; ≤0.2% water (Karl Fischer)
• Perte: ≤0.5% (loss on drying, 110 °C)
• Couleur: white
• pH: 10.0-11.5
• Plage de pH utile: 7-9
• pKa (25 °C): 8.1
• Point d'ébullition: 219-220 °C/10 mmHg (lit.)
• Pf: 167-172 °C (lit.)
• Solubilité: water: soluble (666 mg/ml, yielding a clear colorless solution)
• Absorption: ≤0.05 at 260 at 1 M; ≤0.05 at 290 at 40%; ≤0.06 at 280 at 1 M
• Adéquation: suitable for chromatography; suitable for immunoprecipitation; suitable for molecular biology
• Application(s): clinical research; clinical testing; diagnostic assay manufacturing
• Chaîne SMILES: NC(CO)(CO)CO
• InChI: 1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
• Clé InChI: LENZDBCJOHFCAS-UHFFFAOYSA-N

Description

Description générale

Tris base, also known as Trometamol or Trizma base, plays a crucial role in diverse research applications as a biological buffer. Widely utilized in buffer formulations like TAE and TBE buffers, its pKa of 8.1 renders it well-suited for maintaining pH within the physiological range (pH 7.0-9.0) applicable to most living organisms. Researchers, however, need to be cautious when studying proteins, as Tris may potentially interfere with the activity of certain enzymes.

This compound can be employed independently as a buffer or as part of mixed buffer formulations, including Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, among others. Notably, Tris base is pure, essentially stable, and relatively non-hygroscopic.

In laboratory settings, Tris base proves indispensable for preparing buffers compatible with biological fluids, serving as a standard pH solution, and facilitating various procedures such as lactate dehydrogenase assays, in situ hybridization, and protein extraction from cells. Its versatility extends to applications in cell culture, biochemistry, and molecular biology labs, where it aids in studies related to cell membrane permeability and buffer preparation.

Application

Trizma has been used:

• As a component in the culture of human embryonic stem (hES) cells on human and mouse feeder cells
• To suspend dried protein before SDS-PAGE protein characterization
• As a component of lysis buffer to wash and lyse cells before immunoprecipitation
• Dans l'extraction et l'enrichissement de protéines membranaires en vue d'une séparation par électrophorèse bi-dimensionnelle sur gel de polyacrylamide
• Dans des études de complexes à double brin d'acides nucléiques peptidiques (PNA, peptide nucleic acids) et de leurs séquences d'ADN complémentaires, à l'aide de l'HPLC d'échange d'anions
• En électrochromatographie capillaire et analyse UV des tocophérols et tocotriénols

Caractéristiques et avantages

• Suitable for Cell culture, Biochemical and Molecular Biology Research
• Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
• Effective Buffering from pH 7-9 (25 °C) with a pKa of 8.1 (25 °C)
• Tested for the presence of Anionic and Cationic traces
• Tested for DNase, RNase, and protease

Conditionnement

T6066-1KT :
Chaque kit contient 3 échantillons de 100 g, chaque échantillon étant issu d'un lot de fabrication différent.

Autres remarques

Le pH de tous les tampons dépend de la température et de la concentration. Pour les tampons Tris, le pH augmente de 0,03 unité pour chaque 1 °C de réduction de température, et diminue de 0,03-0,05 unité par dilution d′un facteur dix.
Pour les applications précises, utiliser un pH-mètre soigneusement calibré avec une électrode de verre au calomel.

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Informations légales

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: dust mask type N95 (US), Eyeshields, Gloves