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Key Documents

T3559

Sigma-Aldrich

Anti-γ-Tubulin Antibody

enhanced validation

rabbit polyclonal

Synonyme(s) :

Anti-γ-Tubulin

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

product name

Anti-γ-Tubulin antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution

Source biologique

rabbit

Niveau de qualité

Conjugué

unconjugated

Forme d'anticorps

IgG fraction of antiserum

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

antigen 48 kDa

Espèces réactives

human, chicken

Conditionnement

antibody small pack of 25 μL

Validation améliorée

independent
Learn more about Antibody Enhanced Validation

Technique(s)

indirect immunofluorescence: 1:5,000 using methanol/acetone-fixed chicken fibroblasts
microarray: suitable
western blot: 1:1,000 using cultured A431 whole cell extracts

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

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Description générale

γ-Tubulin (48kDa) is a ubiquitous and highly conserved protein within the microtubule organizing centers (MTOCs) in eukaryotic cells. It is related to α- and β-tubulin and is a member of the tubulin superfamily of proteins. γ-Tubulin abundance is less than 1% of the level of either α- or β-tubulin. γ-Tubulin shares approximately 28-32% identity with α-tubulin from various organisms and 32-36% identity with β-tubulins. Some regions are highly conserved among α-, β- and γ-tubulins.

Spécificité

Anti-gamma-Tubulin recognizes an epitope located in the N-terminal region of gamma-tubulin (amino acids 38-53). Staining of gamma-tubulin (48 kDa) by immunoblotting is specifically inhibited with gamma-tubulin peptide (human, amino acids 38-53 with C-terminally added lysine).

Immunogène

synthetic peptide corresponding to the N-terminal region of human γ-tubulin (amino acids 38-53, with C-terminally added lysine) conjugated to KLH. This sequence is specific for γ-tubulin and not found in other members of the tubulin family such as α− β−, δ and ε− tubulins. This sequence is identical in mouse and rat γ-tubulin and highly conserved among species (Drosophila, Aspergillus, and yeast γ-tubulin).

Application

Anti-γ-Tubulin antibody produced in rabbit has been used in
  • immunofluorescence analysis
  • western blotting
  • immunohistochemistry

Anti-γ-Tubulin polyclonal antibody is suitable for immunoblotting of materials such as A431 cultured cells whole extract (g-tubulin, 48 kDa), and immunocytochemical staining of cells such as cultured chicken fibroblast cells.

Actions biochimiques/physiologiques

γ-Tubulin binds microtubule minus ends and is responsible for mediating the link between microtubules and the centrosome. γ-Tubulin is not a member of microtubule like α- and β-tubulin. It functions as the microtubule nucleator at the microtubule organizing centre (MTOC). It binds to the β-tubulin half of the tubulin molecule, thus establishing the polarity of a microtubule, leaving the α-tubulin half exposed at the plus end.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Ciliary abnormalities due to defects in the retrograde transport protein DYNC2H1 in short-rib polydactyly syndrome
Merrill AE, et al.
American Journal of Human Genetics, 84(4), 542-549 (2009)
A nice ring to the centrosome
Oakley BR
Nature, 378(6557), 555-555 (1995)
Myeloproliferative disorder FOP-FGFR1 fusion kinase recruits phosphoinositide-3 kinase and phospholipase C gamma at the centrosome
Lelievre H, et al.
Molecular Cancer, 7(1), 30-30 (2008)
gamma-Tubulin-gamma-Tubulin Interactions as the Basis for the Formation of a Meshwork
Rossello C, et al.
International Journal of Molecular Sciences, 19(10), 3245-3245 (2018)
ATM is activated by default in mitosis, localizes at centrosomes and monitors mitotic spindle integrity
Oricchio E, et al.
Cell Cycle, 5(1), 88-92 (2006)

Articles

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.

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