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T2315

Sigma-Aldrich

Anti-Tumor Necrosis Factor Soluble Receptor Type II antibody produced in goat

IgG fraction of antiserum, lyophilized powder

Synonyme(s) :

CD120b antigen, Etanercept, TNF sRII, TNF-R2, p75 TNF receptor, p75TBPII, p75TNFR, tumor necrosis factor receptor superfamily member 1B

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About This Item

Numéro MDL:
MFCD01633483
Code UNSPSC :
51111800
Nomenclature NACRES :
NA.41

Source biologique

goat

Niveau de qualité

200

Conjugué

unconjugated

Forme d'anticorps

IgG fraction of antiserum

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

lyophilized powder

Espèces réactives

human

Technique(s)

immunocytochemistry: 5-15 μg/mL (detects TNF RII in human PBLs)
western blot: 1 μg/mL (detects rhs TNF RII at 5 ng/lane under non-reducing conditions)

Numéro d'accès UniProt

P20333

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... TNFRSF1B(7133)

Description générale

TNF RII is a type I transmembrane glycoprotein primarily expressed on hematopoietic cells and is designated TNFRSF1B. TNF RII is a highaffinity receptor for TNF alpha and TNF beta. A soluble form of the receptor generated by proteolytic cleavage functions as a TNF antagonist.

Spécificité

The antibody detects human TNF sRII and shows no cross-reactivity with recombinant human TNF sRI.

Immunogène

recombinant human TNF soluble receptor II (TNF sRII) expressed in Escherichia coli. The immunogen is the non-glycosylated, N-terminal methionyl form of the naturally-occurring human soluble type II receptor for TNF, minus 53 amino acids from the proline-rich region just exterior to the transmembrane domain.

Application

Western Blot: 1 ug/mL detects rhs TNF RII at 5 ng/lane under non-reducing conditions
Immunocytochemistry: Concentration of 5-15 ug/ml detects TNF RII in human peripheral blood lymphocytes

Optimal dilutions should be determined by the end user.

Forme physique

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline (pH 7.4) and 5% trehalose.

Autres remarques

Protein A or G purified

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

13 - Non Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Jason P Twohig et al.
Reviews in the neurosciences, 22(5), 509-533 (2011-08-25)
Tumor necrosis factor receptor superfamily (TNFRSF) members were initially identified as immunological mediators, and are still commonly perceived as immunological molecules. However, our understanding of the diversity of TNFRSF members' roles in mammalian physiology has grown significantly since the first
The Role of Costimulatory Receptors of the Tumour Necrosis Factor Receptor Family in Atherosclerosis
Antunes RF et al
Journal of Biomedicine and Biotechnology, doi: 10-doi: 10 (2012)
B B Aggarwal
Annals of the rheumatic diseases, 59 Suppl 1, i6-16 (2000-10-29)
Tumour necrosis factor (TNF) is a pleiotropic cytokine that mediates apoptosis, cell proliferation, immunomodulation, inflammation, viral replication, allergy, arthritis, septic shock, insulin resistance, autoimmune diseases, and other pathological conditions. TNF transduces these cellular responses through two distinct receptors: type I
M Lotz et al.
Journal of leukocyte biology, 60(1), 1-7 (1996-07-01)
Receptors in the nerve growth factor/tumor necrosis factor receptor family are characterized by the presence of cysteine-rich motifs of approximately 40 amino acids in the extracellular domain. The ligands are type II transmembrane proteins with beta-strands that form a jelly-roll

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