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Key Documents

SRP8045

Sigma-Aldrich

CD40L, Soluble mouse

recombinant, expressed in CHO cells, FLAG® tagged, >95% (SDS-PAGE)

Synonyme(s) :

ACRP30headless:CD154, ACRP30headless:CD40L, ACRP30headless:TNFSF5, ADIPOQ-CD40L

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.32

Source biologique

mouse

Produit recombinant

expressed in CHO cells

Étiquette/Marqueur

FLAG® tagged

Pureté

>95% (SDS-PAGE)

Forme

lyophilized

Poids mol.

35-40 kDa

Conditionnement

pkg of 10 μg

Conditions de stockage

avoid repeated freeze/thaw cycles

Impuretés

<0.01 EU/μg endotoxin, tested

Numéro d'accès UniProt

P27548

Conditions d'expédition

wet ice

Température de stockage

−20°C

Informations sur le gène

mouse ... Cd40lg(21947)

Description générale

CD40L (cluster of differentiation 40 ligand), a member of TNF (tumor necrosis factor) superfamily, is a membrane bound protein. This protein is predominantly expressed transiently by activated CD4+ T cells either as a cell-surface molecule or a secreted multimer protein. Activated B cells, certain DC (dendritic cell) subsets, platelets, and smooth muscle cells also express this protein.

Actions biochimiques/physiologiques

CD40L (cluster of differentiation 40 ligand) interacts with its receptor CD40 which in turn helps mediate the interaction between APCs (antigen presenting cells) and lymphocytes. Activated platelets produce CD40L which then participates in multiple processes such as endothelial cell reactions, formation of germinal center, T-helper cell priming, and cytotoxic T-cell activation. Platelets are also involved in the pathogenesis of asthma through CD40L where they inhibit the production of regulatory T-cells and promote polarization of T-helper cells. Studies in mice show that this protein confers protection against influenza virus, though in a CD4+ T cell independent manner.

Forme physique

Lyophilized from PBS.

Reconstitution

Reconstitute with 100 μL deionized water.

Autres remarques

Mouse CD40L (aa 115-260) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG®-tag.

Informations légales

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Anwar M Hashem et al.
Journal of immunology (Baltimore, Md. : 1950), 193(2), 722-734 (2014-06-15)
CD40L, a key regulator of the immune system, was studied as both a targeting ligand and a molecular adjuvant in nucleoprotein (NP)-based host defense against influenza in mouse models with different genetic backgrounds. Adenoviral vectors secreting NP-CD40L fusion protein (denoted
Jun Tian et al.
Cellular & molecular immunology, 12(6), 700-707 (2014-11-25)
Platelet activation is associated with multiple immune responses and the pathogenesis of various immune-related diseases. However, the exact role and the underlying mechanism of platelets in the progression of allergic asthma remain largely unclear. In this study, we demonstrate that

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