SRP6557

Thrombin Active from human plasma

≥95% (SDS-PAGE), recombinant, lyophilized

• Synonyme(s) : Activated Factor IIa
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.32

Propriétés

• product name: Thrombin Active from human plasma, ≥95% (SDS-PAGE)
• Source biologique: human
• Pureté: ≥95% (SDS-PAGE)
• Forme: lyophilized
• Puissance: >3000 units/mg
• Poids mol.: 37 kDa
• Conditionnement: pkg of 1000 units; pkg of 250 units
• Numéro d'accès UniProt: P00734
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• Informations sur le gène: human ... F2(2147)

Description

Description générale

Thrombin enzyme (activated factor IIa) is an important clotting promoter that controls the transformation of soluble fibrinogen to insoluble active fibrin strands. Thrombin is a coagulation protein and a serine protease (EC 3.4.21.5) that catalyzes many coagulation-related reactions. It is primarily associated with blood clotting.

Actions biochimiques/physiologiques

Thrombin triggers factor-XI, factor-V, Factor-XIII and factor-VIII. Thrombin endorses platelet activation, using activation of protease-activated receptors on the platelet. It also induces inflammation. As a result of its high proteolytic specificity, thrombin has become an important biochemical protein. The thrombin cleavage site (Leu-Val-Pro-Arg-Gly-Ser) is widely used in linker regions of recombinant fusion protein constructs. After the purification of the fusion protein, thrombin is used to cleave between the Arginine and Glycine residues of the cleavage site, efficiently removing the purification tag from the protein of interest with a high degree of specificity.

Forme physique

Lyophilized from a buffer composed of 20 mM Bis-Tris, 150 mM NaCl and 0.1% PEG 8000, pH 6.5.

Reconstitution

A working stock solution can be prepared by adding 1 mL buffered saline, pH 7.4 (ex. 25 mM HEPES, 150 mM NaCl, 0.1% PEG 8000, pH 7.4). Further dilution should be made in buffer containing a suitable stabilizing agent such as 0.1%-1% Prionex, BSA, or PEG.

Clause de non-responsabilité

Ce produit, destiné à la recherche scientifique, est soumis à une réglementation spécifique en France, y compris pour les activités d′importation et d′exportation (Article L 1211-1 alinéa 2 du Code de la Santé Publique). L′acheteur (c′est-à-dire l′utilisateur FINAL) est tenu d′obtenir une autorisation d′importation auprès du ministère français de la recherche, mentionné à l′article L1245-5-1 II du Code de la Santé Publique. En commandant ce produit, vous confirmez détenir l′autorisation d′importation requise.

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H319,H334
• Conseils de prudence: P261 - P264 - P280 - P284 - P305 + P351 + P338 - P337 + P313
• Classification des risques: Eye Irrit. 2 - Resp. Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3