SRP6522

Ubiquitin-TAMRA-labeled (TMR-Ub)

≥95% (SDS-PAGE)

• Synonyme(s) : CEP80, HUBCEP80, RPS27A, Ribosomal Protein S27a, UBA80, UBB, UBCEP1, UBCEP80
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.32

Propriétés

• Pureté: ≥95% (SDS-PAGE)
• Forme: liquid
• Poids mol.: 9.133 kDa
• Conditionnement: pkg of 50 μg
• Concentration: 8 mg/mL
• Conditions d'expédition: dry ice
• Température de stockage: −70°C

Description

Description générale

Post-translational modification of proteins by ubiquitin (Ub) is a key regulatory process that impacts almost all cellular functions. Ubiquitylation occurs through isopeptide linkage between the C-terminus of Ub and the e-amino group of a lysine (Lys) residue on the target substrate [1]. Ub itself has seven Lys residues (6, 11, 27, 29, 33, 48, and 63), any of which can participate in further ubiquitylation, generating polyUb chains [2, 3]. Monitoring the ubiquitylation of target proteins or the growth of polyubiquitin chains has traditionally been carried out with either radiolabeled or epitope-tagged ubiquitin requiring long and laborious detection methods. Fluorescently labeled ubiquitin provides a rapid, facile technique for studying ubiquitin conjugation in vitro. Unlike others, TheTAMRA-labeled ubiquitin carries a single TAMRA molecule attached at a defined location and avoids modification of either the N-terminus or Lys side chains.

Forme physique

≥ 8 mg/mL in PBS.

Informations sur la sécurité

• Code de la classe de stockage: 13 - Non Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable