SRP6241

VAP-1 human

recombinant, expressed in CHO cells, ≥95% (SDS-PAGE)

• Synonyme(s) : AOC3, Copper Amine Oxidase, HPAO, Membrane Primary Amine Oxidase, SSAO, Vascular Adhesion Protein-1
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Produit recombinant: expressed in CHO cells
• Pureté: ≥95% (SDS-PAGE)
• Forme: lyophilized powder
• Poids mol.: ~80 kDa
• Conditionnement: pkg of 10 μg; pkg of 50 μg
• Impuretés: <1 EU/μg endotoxin (LAL test)
• Numéro d'accès UniProt: Q16853
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• Informations sur le gène: human ... VAP1(8639)

Description

Description générale

VAP-1 (vascular adhesion protein 1) is also called as semicarbazide-sensitive amine oxidase. The VAP-1 gene is mapped to human chromosome 17q21.31.

VAP-1 is a type II membrane cell adhesion protein belonging to the copper/topaquinone oxidase family. It is primarily expressed on the high endothelial venules of peripheral lymph nodes and on hepatic endothelia. VAP-1 can catalyze the oxidative deamination of low molecular weight amines, and plays an important role in the migration of lymphocytes to inflamed tissue. Inhibition of VAP-1 can protect against inflammation related damage to certain injured tissues. Additionally, VAP-1 can function as a significant prognostic marker for certain cancers and cardiovascular diseases. Recombinant VAP-1 is a mixture of monomeric and disulfide linked homodimeric forms of a 737 amino acid polypeptide corresponding to amino acids 27 to 763 of the VAP-1 precursor.

Actions biochimiques/physiologiques

VAP-1 (vascular adhesion protein 1) catalyzes an oxygen-dependent reaction involving the conversion of primary amines to aldehyde, ammonia and hydrogen peroxide. Overexpression of VAP-1 is observed in chronic inflammatory diseases such as chronic renal failure, diabetesand autoimmune disorders. VAP-1 mediates the interaction between leukocytes and endothelium in response to inflammation.

Forme physique

Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 7.8.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable