SRP3186

Vitronectin human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture

• Synonyme(s) : Epiboin, S-Protein, Serum-spreading factor, V75, VTN
• Numéro CAS: 83380-82-9
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Niveau de qualité: 200
• Produit recombinant: expressed in HEK 293 cells
• Pureté: ≥95% (HPLC); ≥95% (SDS-PAGE)
• Forme: lyophilized
• Puissance: ≤0.5 μg/mL EC₅₀
• Poids mol.: 52.2 kDa (calculated)
• Conditionnement: pkg of 250 μg
• Technique(s): cell culture | mammalian: suitable
• Impuretés: <0.1 EU/μg endotoxin, tested
• Couleur: white
• Solubilité: water: soluble
• Numéro d'accès NCBI: NM_000638.4
• Numéro d'accès UniProt: P04004
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• Informations sur le gène: human ... VTN(7448)

Description

Description générale

Vitronectin is a secreted glycoprotein which is synthesized in the liver. It is also secreted by respiratory epithelial cells and is present in healthy lungs. It circulates primarily in monomeric form, but can undergo conformational change to a structure that forms disulfide linked multimers.The vitronectin gene consists of approximately 4.5–5 kb base pairs with eight exons and seven introns.
Recombinant human Vitronectin is a protein consisting of 459 amino acids. It exists in two forms: a single chain and a clipped form consisting of two chains held together by disulfide bonds resulting from an endogenous cleavage. Recombinant vitronectin can function as a chemically defined matrix component in human embryonic stem cell renewal media.

Application

Vitronectin human has been used to study the effects of ECM (extracellular matrix) ligands on C6 astroglioma cells. It has also been used as an attachment-promoting protein for coating the cell plates.

Actions biochimiques/physiologiques

The multimeric vitronectin protein can efficiently bind to and incorporate into the extracellular matrix. Within the matrix, Vitronectin can support cell adhesion through binding to various integrins and other proteoglycans. Its interaction with integrin α_vβ₃ enhances hypoxia resistance and tumor cell invasion. It is also associated with cancer progression in various cancers. It is also involved in wound repair and controls complement system as wel. Moreover, vitronectin functions as a marker of profibrotic activity in liver, heart and kidney.

Caractéristiques et avantages

• Human-derived vitronectin
• Produced through recombinant expression in HEK 293 cells
• Low endotoxin levels

Séquence

DQESCKGRCT EGFNVDKKCQ CDELCSYYQS CCTDYTAECK PQVTRGDVFT MPEDEYTVYD DGEEKNNATV HEQVGGPSLT SDLQAQSKGN PEQTPVLKPE EEAPAPEVGA SKPEGIDSRP ETLHPGRPQP PAEEELCSGK PFDAFTDLKN GSLFAFRGQY CYELDEKAVR PGYPKLIRDV WGIEGPIDAA FTRINCQGKT YLFKGSQYWR FEDGVLDPDY PRNISDGFDG IPDNVDAALA LPAHSYSGRE RVYFFKGKQY WEYQFQHQPS QEECEGSSLS AVFEHFAMMQ RDSWEDIFEL LFWGRTSAGT RQPQFISRDW HGVPGQVDAA MAGRIYISGM APRPSLAKKQ RFRHRNRKGY RSQRGHSRGR NQNSRRPSRA TWLSLFSSEE SNLGANNYDD YRMDWLVPAT CEPIQSVFFF SGDKYYRVNL RTRRVDTVDP PYPRSIAQYW LGCPAPGHL

Forme physique

Lyophilized from Phosphate Buffered Saline (PBS), pH 7.4, plus 2.5% trehalose.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Autres remarques

Under reducing conditions, the single chain migrates at an apparent molecular mass of 75kDa on SDS-PAGE, and the clipped form migrates at molecular masses of 65kDa and 10kDa.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable