SRP3075
IL-12p80 human
recombinant, expressed in (BTI-Tn-5B1-4) High-5 Insect Cells, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture
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About This Item
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32
Produits recommandés
Source biologique
human
Produit recombinant
expressed in (BTI-Tn-5B1-4) High-5 Insect Cells
Pureté
≥95% (HPLC)
≥95% (SDS-PAGE)
Forme
lyophilized
Poids mol.
80.0 kDa
Conditionnement
pkg of 10 μg
Technique(s)
cell culture | mammalian: suitable
Impuretés
<0.1 EU/μg endotoxin, tested
Couleur
white to off-white
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Température de stockage
−20°C
Informations sur le gène
human ... IL12B(3593)
Description générale
IL-12 is a disulfide-linked heterodimeric protein (p70), composed of two subunits, p35 and p40, which are encoded by two different genes. Accumulating data indicate that p40 secretion precedes that of IL-12 expression. In addition, to its ability to covalently bind to p35 to form IL-12, p40 can bind to p19 to form IL-23, or can form a homodimer designated as IL-12 p80. Elevated levels of IL-12 p80 are correlated with macrophage recruitment and increased inflammation in asthma and respiratory viral infection models. Recombinant human IL-12 p80 is an 80.0 kDa disulfide linked homodimer consisting of two p40 chains of IL-12.
Actions biochimiques/physiologiques
IL-12 is a disulfide-linked heterodimeric protein (p70), composed of two subunits, p35 and p40, which are encoded by two different genes. Accumulating data indicate that p40 secretion precedes that of IL-12 expression. In addition, to its ability to covalently bind to p35 to form IL-12, p40 can bind to p19 to form IL-23, or can form a homodimer designated as IL-12 p80. Elevated levels of IL-12 p80 are correlated with macrophage recruitment and increased inflammation in asthma and respiratory viral infection models. Recombinant human IL-12 p80 is an 80.0 kDa disulfide linked homodimer consisting of two p40 chains of IL-12.
Séquence
p40 Subunit: IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCS
Forme physique
Lyophilized from 10 mM Sodium Acetate, pH 5.6 + 90 mM NaCl + 2% Sucrose + 4.0% Mannitol.
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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M K Gately et al.
Journal of immunology (Baltimore, Md. : 1950), 147(3), 874-882 (1991-08-01)
IL-12 is a heterodimeric cytokine that was identified on the basis of its ability to synergize with IL-2 in the induction of cytotoxic effector cells and was originally called cytotoxic lymphocyte maturation factor (CLMF). IL-12 was also found to stimulate
M B Atkins et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 3(3), 409-417 (1997-03-01)
A Phase I dose escalation trial of i.v. administered recombinant human interleukin 12 (rhIL-12) was performed to determine its toxicity, maximum tolerated dose (MTD), pharmacokinetics, and biological and potential antineoplastic effects. Cohorts of four to six patients with advanced cancer
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