SRP3063

IGF-Binding Protein 2 human

recombinant, expressed in (BTI-Tn-5B1-4) High-5 Insect Cells, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• Synonyme(s) : IBP-2
• Code UNSPSC : 51111800
• Nomenclature NACRES : NA.77

Propriétés

• Source biologique: human
• Produit recombinant: expressed in (BTI-Tn-5B1-4) High-5 Insect Cells
• Pureté: ≥98% (HPLC); ≥98% (SDS-PAGE)
• Forme: lyophilized
• Poids mol.: 31.5 kDa
• Conditionnement: pkg of 20 μg
• Technique(s): cell culture | mammalian: suitable
• Impuretés: <0.1 EU/μg endotoxin, tested
• Couleur: white to off-white
• Numéro d'accès UniProt: P18065
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• Informations sur le gène: human ... IGFBP2(3485)

Description

Description générale

IGFBPs (insulin-like growth factor-binding proteins) are mainly required to regulate IGFs (insulin-like growth factors) activities. IGFBP-2 belongs to the IGFBP family of proteins. The protein has a IGF-binding site and a thyroglobulin type-1 domain. It interacts with IGF1 and IGF2. IGFBP-2 is widely expressed in tissues. This gene is mapped to human chromosome 2q35.
Recombinant human IGF-BP2 is a 31.5kDa protein consisting of 289 amino acid residues including the IGF-BP domain and thyroglobulin type-I domain.

Application

IGF-Binding Protein 2 human has been used to study the vaccine-stimulated augmented immunity in the presence of IGFBP-2 in patients with metastatic breast cancer.

Actions biochimiques/physiologiques

IGFBP-2 (insulin-like growth factor-binding protein 2) interacts with integrins and extracellular matrix via arginine-glycine-aspartic acid (RGD) and heparin-binding motifs. It participates in cell detachment, migration and invasion. IGFBP-2 is also associated with various malignancies, including ovarian cancer, breast cancer, colon cancer, lung cancer, prostate cancer, glioma and leukemia. It is suggested as a serum biomarker of Lupus Nephritis and is associated with the chronicity of renal pathology in patients. IGFBP-2 is upregulated in idiopathic pulmonary fibrosis.

Séquence

EVLFRCPPCT PERLAACGPP PVAPPAAVAA VAGGARMPCA ELVREPGCGC CSVCARLEGE ACQVYTPRCG QGLRCYPHPG SELPLQALVM GEGTCEKRRD AEYGASPEQV ADNGDDHSEG GLVENHVDST MNMLGGGGSA GRKPLKSGMK ELAVFREKVT EQHRQMGKGG KHHLGLEEPK KLRPPPARTP CQQELDQVLE RISTMRLPDE RGPLEHLYSL HIPNCDKHGL YNLKQCKMSL NGQRGECWCV NPNTGKLIQG APTIRGDPEC HLFYNEQQEA RGVHTQRMQ

Forme physique

Lyophilized from 1x PBS, pH 7.2.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable