SRP0706

KAT5 (TIP60), Active

recombinant

• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.77

Propriétés

• Produit recombinant: expressed in baculovirus infected Sf9 cells
• Pureté: 70-95% (SDS-PAGE)
• Forme: frozen liquid
• Activité spécifique: 25-35 nmol/min·mg (radiometric)
• Poids mol.: ~88 kDa
• Concentration: 0.1 μg/μL
• Numéro d'accès: NM_006388
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... KAT5(10524)

Description

Application

Useful for acetyltransferase assay.

Actions biochimiques/physiologiques

Full-length recombinant human KAT5 was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. KAT5 or K (lysine) acetyltransferase 5 is a signaling protein that belongs to the MYST family of histone acetyl transferases (HATs). KAT5 was originally isolated as an HIV-1 TAT-interactive protein that plays an important role in regulating chromatin remodeling, transcription and other nuclear processes by acetylating histone and nonhistone proteins ⁽¹⁾. KAT5 protein is a histone acetylase that is involved in DNA repair and apoptosis and plays an important role in signal transduction. KAT5 acts as a cofactor of TAT that is involved in the regulation of HIV gene expression. KAT5 is also a co-regulator of transcription factors that either promote or suppress tumorigenesis ⁽²⁾.

Reconstitution

Formulated in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

Stockage et stabilité

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable