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Key Documents

SRP0395

Sigma-Aldrich

SETD2 active human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

Synonyme(s) :

HYPB, SET domain containing 2, SET2

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.32

Source biologique

human

Produit recombinant

expressed in E. coli

Pureté

≥70% (SDS-PAGE)

Forme

aqueous solution

Poids mol.

60.9 kDa

Conditionnement

pkg of 50 μg

Numéro d'accès NCBI

NM_014159

Numéro d'accès UniProt

Q9BYW2

Conditions d'expédition

dry ice

Température de stockage

−70°C

Informations sur le gène

human ... SETD2(29072)

Description générale

SET domain containing 2 (SETD2) is a histone methyltransferase gene and is mapped on human chromosome 3p21.31, a region associated with growth arrest of cancer cells.
Human SETD2/pGEX6P1 (GenBank Accession No. NM_014159), amino acids 1418-1714 with N-terminal GST-tag, MW= 60.9kDa, expressed in an Escherichia coli expression system.

Actions biochimiques/physiologiques

SET domain containing 2 (SETD2) plays a vital role in trimethylation of the histone mark H3K36 (histone 3 lysine 36). Mutation of this tumor suppressor gene (TSG) is associated with the pathogenesis of various cancers including, clear cell renal cell carcinoma (cRCC), breast cancer, lung cancer, acute lymphoblastic leukemia(ALL), and glioma. In addition, SETD2 gene is also involved in the development of Huntington′s disease (HD). SETD2 plays an important role in the transcriptional elongation by maintaining chromatin structure through association with hyperphosphorylated RNA polymerase II (POLR2A). SETD2 interacts with p53 and act as a tumor suppressor in breast cancer cells. SETD2 is implicated in DNA double-strand break (DSB) exclusion and homologous recombination (HR) repair in human cells. SETD2 in HeLa cells alters nucleosome organization via decreasing the recruitment of the FACT (facilitates chromatin transcription) complex to active genes.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Histone methyltransferase gene SETD2 is a novel tumor suppressor gene in clear cell renal cell carcinoma.
Duns G
Cancer Research, 70(11), 4287-4291 (2010)
The mRNA expression of SETD2 in human breast cancer: correlation with clinico-pathological parameters.
Al Sarakbi W
BMC Cancer, 9:290 (2009)
Histone methyltransferase SETD2 coordinates FACT recruitment with nucleosome dynamics during transcription.
Carvalho S
Nucleic Acids Research, 41(5), 2881-2893 (2013)
SETD2-dependent histone H3K36 trimethylation is required for homologous recombination repair and genome stability.
Pfister SX
Cell Reports, 7(6), 2006-2018 (2014)
S Rega et al.
Molecular and cellular neurosciences, 18(1), 68-79 (2001-07-20)
Neurodegeneration in Huntington's disease (HD) is associated with an elongated glutamine tract in the widely expressed huntingtin protein. Although the pathogenic mechanisms are still unknown, the distinct physical properties of mutant huntingtin in the brain suggest that other factors including
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