Accéder au contenu
MilliporeSigma
Toutes les photos(2)

Key Documents

SRP0189

Sigma-Aldrich

Pyruvate kinase M2 Active from mouse

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

Synonyme(s) :

M2-PK tumor, OPA-interacting protein 3 (OIP-3), Pyruvate kinase 3 (PK3), Pyruvate kinase, muscle, Thyroid hormone-binding protein 1 (THBP1)

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme


About This Item

Code UNSPSC :
12352204
Nomenclature NACRES :
NA.32

Source biologique

mouse

Produit recombinant

expressed in E. coli

Pureté

≥90% (SDS-PAGE)

Forme

aqueous solution

Poids mol.

58 kDa

Conditionnement

pkg of 20 μg

Conditions de stockage

avoid repeated freeze/thaw cycles

Concentration

>0.02 mg/mL

Numéro d'accès NCBI

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Température de stockage

−70°C

Informations sur le gène

mouse ... PKM2(18746)

Description générale

Pyruvate kinase M2 (PKM2), also known as pyruvate kinase 3 (PK3), is an isoform of pyruvate kinase that is expressed in some differentiated tissues, such as lung, fat, retina, and pancreatic islets, as well as in normal proliferating cells and embryonic cells. PKM2/pk3 is encoded by the gene mapped to mouse chromosome 9.
Murine Pyruvate kinase M2 (PKM2) (GenBank Accession No. NM_011099), amino acids 2-end, with N-terminal His tag, MW = 58kDa, expressed in an Escherichia coli expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Actions biochimiques/physiologiques

Elevated expression of PKM2 is observed in most of the cancer cells. Hence, this protein can be considered as a potent therapeutic target for cancers. PKM2 plays an important role in the discharge of excessive intracellular reactive oxygen species (ROS), which otherwise damage cellular components and decrease cell viability. Protein kinase activity of PKM2 is involved in the regulation of gene expression and tumorigenesis. PKM2 is an important rate-limiting enzyme involved in final step of glycolysis and it has an essential role in metabolic reprogramming throughout cancer progression.

Définition de l'unité

One unit is defined as the amount of enzyme required to transfer a phosphoryl group from phosphoenolpyruvate (PEP) to 1 pmol of ADP/min at 37°C

Forme physique

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

Notes préparatoires

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

PKM2 and cancer: The function of PKM2 beyond glycolysis.
Dong G
Oncology Letters, 11(3), 1980-1986 (2016)
Polymorphism of kidney pyruvate kinase in the mouse is determined by a gene, Pk-3, on chromosome 9.
Peters J
Biochemical Genetics, 19(7-8), 757-769 (1981)
Weiwei Yang et al.
Cancer letters, 339(2), 153-158 (2013-06-25)
Altered metabolism is fundamental to the growth and survival of cancer cells. Pyruvate kinase M2 (PKM2), a key enzyme in cancer metabolism, has been demonstrated to play a central role not only in metabolic reprogramming but also in direct regulation

Articles

We presents an article about the Warburg effect, and how it is the enhanced conversion of glucose to lactate observed in tumor cells, even in the presence of normal levels of oxygen. Otto Heinrich Warburg demonstrated in 1924 that cancer cells show an increased dependence on glycolysis to meet their energy needs, regardless of whether they were well-oxygenated or not.

We presents an article about the Warburg effect, and how it is the enhanced conversion of glucose to lactate observed in tumor cells, even in the presence of normal levels of oxygen. Otto Heinrich Warburg demonstrated in 1924 that cancer cells show an increased dependence on glycolysis to meet their energy needs, regardless of whether they were well-oxygenated or not.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique