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Principaux documents

SRP0183

Sigma-Aldrich

Hsp90b Active human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

Synonyme(s) :

HSP84, HSP90Beta, HSPC2, Heat shock protein 90 kDa β

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.32

Source biologique

human

Produit recombinant

expressed in E. coli

Essai

≥80% (SDS-PAGE)

Forme

aqueous solution

Poids mol.

83 kDa

Conditionnement

pkg of 200 μg

Concentration

>0.02 mg/mL

Numéro d'accès NCBI

AY359878

Numéro d'accès UniProt

P08238

Conditions d'expédition

dry ice

Température de stockage

−70°C

Informations sur le gène

human ... HSP90AB1(3326)

Description générale

Hsp90b (heat shock protein 90-β) is a constitutive cytosolic isoform of Hsp90. The gene is mapped to human chromosome 6p21.1. Hsp90b is an ATP-dependent molecular chaperone and is upregulated in many tumor cells.
Human Heat Shock Protein 90β (GenBank Accession No. AY359878), full length with C-terminal His tag, MW = 83kDa, expressed in an Escherichia coli expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Actions biochimiques/physiologiques

Hsp90 (heat shock protein 90) is needed for folding, maturation, transport, and degradation of various proteins, particularly signaling molecules, steroid receptors, and transcription factors. Hsp90b (Hsp90 β) is secreted by various cells and works as a chaperone for selected surface-associated or secreted proteins. It acts as a chaperone for matrix metalloproteinases and extracellular matrix proteins, and helps in cell motility. Suppression of the Hsp90b activity is suggested as a therapeutic approach for various cancers and inflammatory diseases.

Forme physique

Formulated in 25 mM Tris-HCl, pH 7.5, 100mM NaCl, 0.05% Tween 20, 20% glycerol and 3 mM DTT.

Notes préparatoires

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

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Certificats d'analyse (COA)

Lot/Batch Number

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Consulter la Bibliothèque de documents

Cell surface heparan sulfate proteoglycans are involved in the binding of Hsp90a and Hsp90? to the cell plasma membrane.
Snigireva AV, et al.
Cell adhesion & migration, 9, 460-460 (2015)
[Hsp90AB1 Protein is Overexpressed in Non-small Cell Lung Cancer Tissues ?and Associated with Poor Prognosis in Lung Adenocarcinoma Patients].
Wang M, et al.
Zhongguo Fei ai za Zhi = Chinese Journal of Lung Cancer, 19, 64-64 (2016)
DNA copy number analysis of Grade II-III and Grade IV gliomas reveals differences in molecular ontogeny including chromothripsis associated with IDH mutation status.
Cohen A, et al.
Acta Neuropathologica Communications, 3, 34-34 (2015)
HSP90AB1: Helping the good and the bad.
Haase M, et al.
Gene, 575, 171-171 (2016)

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