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Key Documents

SRP0113

Sigma-Aldrich

HDAC-11 human

recombinant, expressed in baculovirus infected insect cells, ≥10% (SDS-PAGE)

Synonyme(s) :

HD11, Histone deacetylase 11

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.77

Source biologique

human

Produit recombinant

expressed in baculovirus infected insect cells

Pureté

≥10% (SDS-PAGE)

Forme

aqueous solution

Poids mol.

66 kDa

Conditionnement

pkg of 50 μg

Conditions de stockage

avoid repeated freeze/thaw cycles

Concentration

>0.02 mg/mL

Numéro d'accès NCBI

BC009676

Numéro d'accès UniProt

Q96DB2

Conditions d'expédition

dry ice

Température de stockage

−70°C

Informations sur le gène

human ... HDAC11(79885)

Description générale

HDAC-11 (histone deacetylase 11) is a zinc-dependent HDAC. It belongs to the HDAC family of proteins and HDAC class IV. It is highly conserved among various species. The mRNA is present in the brain, skeletal muscle, kidney and testis. The gene is mapped to human chromosome 3p25.
Human HDAC11, full length, GenBank Accession No. BC009676, with N-terminal GST tag, MW = 66kDa, expressed in baculovirus expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Actions biochimiques/physiologiques

HDAC-11 (histone deacetylase 11) is responsible for deacetylation of nuclear histone and nonhistone proteins, including transcription factors. HDAC-11 catalyzes the deacetylation of histone H4. It binds to CDT1 (DNA replication factor) and is present with S phase-associated replication origins. HDAC-11 is upregulated in various carcinomas. Absence of it results in cell death and suppresses metabolic activity in colon, prostate, breast and ovarian cancer cell lines.

Définition de l'unité

One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C.

Forme physique

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 50% glycerol.

Notes préparatoires

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Chromatin unfolding by Cdt1 regulates MCM loading via opposing functions of HBO1 and HDAC11-geminin.
Wong PG, et al.
Cell Cycle, 9, 4351-4351 (2010)
HDAC11 plays an essential role in regulating OX40 ligand expression in Hodgkin lymphoma.
Buglio D, et al.
Blood, 117, 2910-2910 (2011)
Chromosomal organization and localization of the novel class IV human histone deacetylase 11 gene.
Voelter-Mahlknecht S, et al.
International Journal of Molecular Medicine, 16, 589-589 (2005)
HDAC11 is a novel drug target in carcinomas.
Deubzer HE, et al.
International Journal of Cancer. Journal International Du Cancer, 132, 2200-2200 (2013)

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