SAE0069

Cholera Toxin B subunit

≥95% (SDS-PAGE), lyophilized powder, enterotoxin

• Synonyme(s) : CTB, CTxB, Cholera enterotoxin B chain, Cholera enterotoxin gamma chain, Cholera enterotoxin subunit B, Choleragenoid, ToxB
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.32

Propriétés

• product name: Cholera Toxin B subunit, recombinant, expressed in HEK 293 cells, subunit A free
• Produit recombinant: expressed in HEK 293 cells
• Niveau de qualité: 200
• Pureté: ≥95% (SDS-PAGE)
• Forme: lyophilized powder
• Activité spécifique: (Binds to GM1 ganglioside. The binding EC50 is lower than 1 ug/ml)
• Poids mol.: pentamer observed mol wt ~70 kDa by SDS-PAGE (non-reducing); calculated mol wt 13 kDa; observed mol wt ~13 & 20 kDa by SDS-PAGE (reducing)
• Solubilité: H₂O: soluble 11 mg/mL
• Numéro d'accès UniProt: P01556
• Température de stockage: −20°C

Description

Description générale

The advantage of this product over the native CTXB is that it has no traces of Vibrio cholera, and no contamination of the highly toxic Cholera toxin A subunit. This product is expressed in human HEK 293 cells as a C-terminally his-tagged protein with a calculated molecular mass of 13 kDa (amino acids Thr22-Asn124).

The protein is recombinant, and therefore has no contamination of the highly toxic Cholera toxin A subunit.

Under non-reducing conditions, it migrates as pentamer of ~70 kDa, consistent with the pentamerization of CTXB.

Application

Cholera Toxin B subunit has been used:

• for macrophage stimulation and i.p. injection in a study to determine the endotoxin sensitivity of Caspase-4.
• in transganglionic and retrograde tract-tracing method combined with dual-immunofluorescence histochemistry of adult rat Vmes neuron cells.

Forme physique

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.

Reconstitution

When reconstituted with water to a final concentration of 1 mg of CTB per ml, the solution will contain 1X PBS

Remarque sur l'analyse

Activity measured by ELISA using ganglioside GM1-coated multiwell plates, rabbit anti-Cholera toxin B subunit antibodies, and peroxidase-labeled goat anti-rabbit IgG as the secondary antibody. 50% saturation of binding was achieved with ≤1 μg of Cholera toxin B subunit per mL.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable