SAB4702134

Monoclonal Anti-CD3 PE produced in mouse

• Synonyme(s) : CD3E, T3E, TCRE
• Code UNSPSC : 12352203

Propriétés

• Conjugué: phycoerythrin (R-PE) conjugate
• Niveau de qualité: 100
• Type de produit anticorps: primary antibodies
• Clone: SK7, monoclonal
• Forme: buffered aqueous solution
• Espèces réactives: (Non-human primates), ( Human)
• Technique(s): flow cytometry: suitable
• Isotype: IgG1κ
• Numéro d'accès UniProt: P07766
• Température de stockage: 2-8°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... CD3E(916)

Description

Description générale

CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkynje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.

Spécificité

The mouse monoclonal antibody SK7 recognizes an extracellular epitope on the CD3 antigen on mature human T cells. This antibody reacts with the epsilon chain of the CD3 complex. The monoclonal antibodies SK7 and UCHT1 recognize overlapping epitopes.

Immunogène

Human thymocytes

Forme physique

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Notes préparatoires

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Stockage et stabilité

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Autres remarques

The antibody solution should be gently mixed before use.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable