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SAB4504014

Sigma-Aldrich

Anti-phospho-Akt (pTyr474) antibody produced in rabbit

affinity isolated antibody

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

antigen 55 kDa

Espèces réactives

human, mouse, rat

Concentration

~1 mg/mL

Technique(s)

ELISA: 1:5000
immunohistochemistry: 1:50-1:100
western blot: 1:500-1:1000

Numéro d'accès NCBI

P31749

Numéro d'accès UniProt

P31749

Conditions d'expédition

wet ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

phosphorylation (pTyr474)

Informations sur le gène

human ... AKT1(207)

Description générale

The AKT1 (AKT serine/threonine kinase 1) gene is also called as v-akt murine thymoma viral oncogene homolog 1 and PKBα (protein kinase B). It is upregulated in liver, muscle and adipocytes. AKT1 gene is mapped to human chromosome 14q32. It is a member of AKT family that has an amino-terminal pleckstrin homology (PH) domain, a short α-helical linker and a carboxyl-terminal kinase domain.

Immunogène

The antiserum was produced against synthesized peptide derived from human Akt around the phosphorylation site of Tyr474.

Immunogen Range: 431-480

Actions biochimiques/physiologiques

The protein encoded by AKT1 (AKT serine/threonine kinase 1) gene controls the survival of cells and anti-apoptotic actions that attack the pathogenesis of several cancers, hence it plays a crucial role in tumorigenesis. In mice, AKT1 is essential for normal development.

Caractéristiques et avantages

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Forme physique

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Certificats d'analyse (COA)

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Association between single nucleotide polymorphisms in AKT1 and the risk of prostate cancer in the Chinese Han population
Liu JM, et al.
Genetics and molecular research : GMR (2017)
Akt1/PKBalpha is required for normal growth but dispensable for maintenance of glucose homeostasis in mice
Cho H, et al.
The Journal of Biological Chemistry, 276(42), 38349-38352 (2001)
AKT plays a central role in tumorigenesis
Testa JR and Bellacosa A
Proceedings of the National Academy of Sciences of the USA, 98(20), 10983-10985 (2001)
Christiane E Koch et al.
Endocrinology, 155(5), 1806-1816 (2014-02-26)
Adiponectin, an adipocyte-derived hormone, regulates glucose and lipid metabolism. It is also antiinflammatory. During obesity, adiponectin levels and sensitivity are reduced. Whereas the action of adiponectin in the periphery is well established the neuroendocrine role of adiponectin is largely unknown.
Lingxiang Yu et al.
Cancer epidemiology, 38(4), 408-413 (2014-06-14)
Previous evidence has shown that microRNA (miR)-224 may function as an onco-miRNA in hepatocellular carcinoma (HCC) cells by activating AKT signaling. However, little is known about the clinical significance of the combined expression of miR-224 and phosphorylated-AKT (pAKT) on human
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