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SAB4200762

Sigma-Aldrich

Anti-Opsin antibody, Mouse monoclonal

clone RET-P1, purified from hybridoma cell culture

Synonyme(s) :

Anti-Opsin 2, Anti-Rhodopsin

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

200

Forme d'anticorps

purified from hybridoma cell culture

Type de produit anticorps

primary antibodies

Clone

RET-P1, monoclonal

Forme

buffered aqueous solution

Poids mol.

~39 kDa

Espèces réactives

avian, bovine, fish, mouse, rat, human, amphibian, turtle, rabbit

Concentration

~1 mg/mL

Technique(s)

ELISA: suitable
immunoblotting: 2-4 μg/mL using human retinoblastoma Y79 cell line extract.
immunofluorescence: suitable
immunohistochemistry: 5-10 μg/mL using rat eye frozen sections.
radioimmunoassay: suitable

Isotype

IgG1

Numéro d'accès UniProt

P08100

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

rat ... Rho(24717)

Description générale

Anti-Opsin antibody, Mouse monoclonal (rhodopsin) (mouse IgG1 isotype) is derived from the RET-P1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/C mice immunized with rat retinal membranes. Opsin is also called as rhodopsin protein, members of the G-protein coupled receptor (GPCR) superfamily. Opsin comprises >95% of the rod outer segments (ROS) intrinsic membrane and consists of a protein moiety - an opsin - and a non-protein moiety - the chromophore retinal.

Application

Anti-Opsin antibody has been used in
  • immunoblotting
  • immunofluorescence
  • immunohistochemistry
  • enzyme linked immunosorbent assay (ELISA)
  • radioimmuno assay (RIA)

Actions biochimiques/physiologiques

Opsin is an essential molecules for mediating the ability of animals to detect and use light for diverse biological functions. Mutations in the opsin (rhodopsin) gene are linked to retinitis pigmentosa (RP), a disease characterized by retinal degeneration resulting in reduced peripheral vision and night blindness.

Autres remarques

rat retinal membranes

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

The opsins
Terakita A.
Genome Biology, 6(3), 213-213 (2005)
Retinitis pigmentosa
Hamel C.
Orphanet Journal of Rare Diseases, 1(1), 40-40 (2006)
Yuanyuan Chen et al.
The Journal of biological chemistry, 289(13), 9288-9303 (2014-02-12)
The P23H opsin mutation is the most common cause of autosomal dominant retinitis pigmentosa. Even though the pathobiology of the resulting retinal degeneration has been characterized in several animal models, its complex molecular mechanism is not well understood. Here, we
P A Hargrave et al.
Experimental eye research, 42(4), 363-373 (1986-04-01)
Antisera and monoclonal antibodies to rhodopsin were examined for their binding specificity to rhodopsin by using peptides from the rhodopsin sequence as competitors for antibody binding to rhodopsin in an enzyme-linked immunoassay. Monoclonal antibodies tested were raised in mice against
T Watanabe et al.
Development (Cambridge, England), 114(4), 899-906 (1992-04-01)
We previously developed a reaggregate cell culture system in which embryonic rat retinal neuroepithelial cells proliferate and give rise to opsin-expressing rod photoreceptor cells (rods) on the same schedule in vitro as they do in vivo. We showed that the
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