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SAB4200709

Sigma-Aldrich

Anti-Collagen Type IV antibody, Mouse monoclonal

clone col-94, purified from hybridoma cell culture

Synonyme(s) :

Anti-col4a1/col4a2

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Forme d'anticorps

purified from hybridoma cell culture

Type de produit anticorps

primary antibodies

Clone

col-94, monoclonal

Forme

buffered aqueous solution

Espèces réactives

monkey, human

Concentration

~1 mg/mL

Technique(s)

dot blot: suitable
electron microscopy: suitable
immunohistochemistry: 4-8 μg/mL using formalin-fixed, paraffin-embedded human tongue sections

Isotype

IgG1

Numéro d'accès UniProt

P02462

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... COL4A1(1282)

Description générale

Anti-Collagen Type IV antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the col-94 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse immunized with human collagen Type IV. Collagen Type IV (also known as COL4A) is a key component of the basement membranes (BM), which is found at the basal surface of epithelial and endothelial cells and is essential for tissue strength and polarity. Type IV Collagens are encoded by three pairs of paralogous genes (COL4A1 through COL4A6).

Immunogène

human collagen Type IV

Application

Anti-Collagen Type IV antibody, Mouse monoclonal has been used in immunohistochemistry and dot-blotting.

Actions biochimiques/physiologiques

Collagen Type IV represents a physical barrier against cell invasion. Proteolysis/degradation of Collagen Type IV is essential for cell migration and cancer cells invasion through the ECM into the interstitial tissues.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Raghu Kalluri
Nature reviews. Cancer, 3(6), 422-433 (2003-06-05)
In recent years, the basement membrane (BM)--a specialized form of extracellular matrix (ECM)--has been recognized as an important regulator of cell behaviour, rather than just a structural feature of tissues. The BM mediates tissue compartmentalization and sends signals to epithelial
Dynamic interplay between the collagen scaffold and tumor evolution
Egeblad M, et al.
Current Opinion in Cell Biology, 22(5), 697-706 (2010)
Debbie S Kuo et al.
Human molecular genetics, 21(R1), R97-110 (2012-08-24)
Heterotrimers composed of collagen type IV alpha 1 (COL4A1) and alpha 2 (COL4A2) constitute one of the most abundant components of nearly all basement membranes. Accordingly, mutations in COL4A1 or COL4A2 are pleiotropic and contribute to a broad spectrum of
R Grant Rowe et al.
Trends in cell biology, 18(11), 560-574 (2008-10-14)
The basement membrane (BM), a specialized network of extracellular matrix macromolecules, surrounds epithelial, endothelial, muscle, fat and nerve cells. During development, immune surveillance and disease states ranging from cancer to fibrosis, host cells penetrate the BM by engaging tissue-invasive programs
Characterization and isolation of poly- and monoclonal antibodies against collagen for use in immunohistochemistry.
S Gay et al.
Methods in enzymology, 145, 148-167 (1987-01-01)
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