SAB4200697

Anti-Tyrosine Hydroxylase antibody, Mouse monoclonal

clone TH-16, purified from hybridoma cell culture

• Synonyme(s) : TH, TYH
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.44

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: TH-16, monoclonal
• Forme: buffered aqueous solution
• Espèces réactives: rat, dog, rabbit, human, sheep, guinea pig, mouse, monkey, bovine
• Concentration: ~1 mg/mL
• Technique(s): immunoblotting: 1-2 μg/mL using whole extract of rat PC-12 cells; immunohistochemistry: suitable; immunoprecipitation (IP): suitable
• Isotype: IgG1
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... TH(7054)

Description

Description générale

Monoclonal Anti-tyrosine hydroxylase (mouse IgG1 isotype) is derived from the hybridoma TH-16 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified rat tyrosine hydroxylase. This antibody recognizes an epitope located in the N-terminal region of human, monkey, bovine, sheep, rabbit, dog, guinea pig, mouse and rat tyrosine hydroxylase (TH).
Tyrosine hydroxylase (TH) is encoded by the gene mapped to human chromosome 11p15. TH is a tetramer of four identical subunits, which is characterized with a regulatory, catalytic, and tetramerization domains. The enzyme utilizes tyrosine, BH4 and O₂ as co-substrates, and Fe²⁺ as a cofactor.

Immunogène

purified rat Tyrosine Hydroxylase

Actions biochimiques/physiologiques

Tyrosine hydroxylase (TH) catalyzes the first rate limiting step in the biosynthesis of catecholamine neurotransmitter,that is, the conversion of L-tyrosine to L-dopa. Inhibition of TH by L-phenylalanine, might play a crucial role in phenylketonuria and block the synthesis of norepinephrine. Activity of TH can be regulated by phosphorylation. Decreased expression of TH is associated with various neuro-psychiatric diseases such as schizophrenia and Parkinson′s disease (PD).

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Stockage et stabilité

For continuous use, store at 2–8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation. Working dilution samples should be discarded if not used within 12 hours.

Autres remarques

In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable