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SAB4200676

Sigma-Aldrich

Monoclonal Anti-SPAG11 antibody produced in mouse

clone 10B4, purified from hybridoma cell culture

Synonyme(s) :

Anti-Spag11, Anti-Spag11a/b, Bin1b, sperm associated antigen 11A/B

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About This Item

Code UNSPSC :
51111800
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

200

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

10B4, monoclonal

Forme

buffered aqueous solution

Poids mol.

monomer ~7 kDa
 ~34 kDa (multimer)

Espèces réactives

rat, mouse

Concentration

~1 mg/mL

Technique(s)

immunoblotting: 4-8 μg/mL using whole extracts of HEK-293 cells overexpressing rat SPAG11
immunohistochemistry: 5-10 μg/mL using paraffin-embedded rat caput epididimys sections

Isotype

IgG1

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

mouse ... Spag11a(78128)
rat ... Spag11a(246305)

Description générale

Monoclonal Anti-SPAG11 (mouse IgG1 isotype) is derived from the hybridoma 10B4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. SPAG11 is expressed specifically in epithelial cells in the middle part of the caput region. It exists in four isoforms such as A, D, E and G.
SPAG11A (sperm associated antigen 11A) belongs to the β defensin family. The protein is mainly expressed in the epididymis. The gene is mapped to human chromosome 8.

Spécificité

Monoclonal Anti-SPAG11 recognizes mouse and rat SPAG11.

Immunogène

a recombinant rat SPAG11

Application

Monoclonal Anti-SPAG11 antibody produced in mouse may be used in immunoblotting and immunohistochemistry.

Actions biochimiques/physiologiques

SPAG11 is involved in promoting sperm motility due to its capability to bind the sperm head in different regions of the epididymis. It exhibits anti-microbial activity by suppressing colony growth of Escherichia coli and other Gram-negative bacteria.
SPAG11A (sperm associated antigen 11A) is a secretory protein with possible involvement in sperm maturation. It also controls inflammatory responses in the epididymis.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Stockage et stabilité

For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing or storage in “frost-free” freezer is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Identification, cloning and functional characterization of novel sperm associated antigen 11 (SPAG11) isoforms in the rat
Yenugu S, et al.
Reproductive Biology and Endocrinology, 4(1), 1-14 (2006)
Kumari Sangeeta et al.
Cell and tissue research, 379(3), 601-612 (2019-11-07)
Differential expression of a variety of proteins in the four major regions of the epididymis contributes to maturation of spermatozoa and region-specific cellular functions as well. Proliferation of epithelial cells of the epididymis is highly controlled and thus is one
Dwi A Pujianto et al.
Reproductive biology and endocrinology : RB&E, 11, 59-59 (2013-07-03)
Epididymal sperm maturation occurs via interactions between sperm and proteins secreted by the epididymal epithelium. Although this is an important process, the genes that encode the involved proteins remain largely uncharacterized. Previous studies have demonstrated that the genes involved in
Multimerin-2 maintains vascular stability and permeability
Pellicani R, et al.
Matrix Biology, 87, 11-25 (2020)
Chen Xi Zhou et al.
Nature cell biology, 6(5), 458-464 (2004-05-04)
Although the role of the epididymis, a male accessory sex organ, in sperm maturation has been established for nearly four decades, the maturation process itself has not been linked to a specific molecule of epididymal origin. Here we show that
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