SAB4200202

Anti- INI1/SNF5 antibody, Mouse monoclonal

clone 2C2, purified from hybridoma cell culture

• Synonyme(s) : Anti-BAF47, Anti-RDT, Anti-RTPS1, Anti-SMARCB1, Anti-SNF5L1, Anti-SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1, Anti-Sfh1p, Anti-Snr1, Anti-hSNFS
• Numéro MDL: MFCD04118401
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Conjugué: unconjugated
• Forme d'anticorps: purified from hybridoma cell culture
• Type de produit anticorps: primary antibodies
• Clone: 2C2, monoclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen ~44 kDa
• Espèces réactives: monkey, bovine, canine, mouse, rat, human
• Concentration: ~1.0 mg/mL
• Technique(s): immunocytochemistry: suitable; western blot: 1.0-2.0 μg/mL using HeLa cell extracts
• Isotype: IgG2a
• Numéro d'accès UniProt: Q12824
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... SMARCB1(6598)

Description

Description générale

INI1/SNF5, also known as SWItch/sucrose non-fermentable (SWI/SNF) related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1, is encoded by the gene mapped to human chromosome 22q11.2. This gene codes for an essential component of chromatin-remodelling SWI/SNF multiprotein complexes.

Monoclonal Anti-INI1/SNF5 (mouse IgG2a isotype) is derived from the hybridoma 2C2 produced by the fusion of mouse myeloma cells (SP2) and splenocytes from mouse immunized with a human INI1 fusion protein. Integrase interactor 1 (INI1)/sucrose non-fermentable 5 (SNF5) binds to human immunodeficiency virus type 1 (HIV-1) integrase.

Immunogène

human INI1 fusion protein.

Application

Monoclonal Anti-INI1/SNF5 antibody produced in mouse has been used in immunoblotting and immunocytochemistry.

Actions biochimiques/physiologiques

INI1/sucrose non-fermentable 5 (SNF5) plays a vital role in transcriptional activation of inducible genes through chromatin remodelling. Loss of function mutations of the gene is associated with the pathogenesis of aggressive pediatric cancer and epithelioid schwannoma. The encoded protein triggers DNA-joining activity of the HIV-1 integrase in vitro. Additionally, it might also help in targeting the viral DNA to active genes. SNF5 interacts with E1 protein of human papillomavirus (HPV) and thus, enhances the efficiency of HPV DNA replication.

Integrase interactor 1 (INI1)/sucrose non-fermentable 5 (SNF5) is recruited to HIV-1 preintegration complexes before nuclear migration and represses the basal human immunodeficiency virus type 1 promoter activity. This gene is a tumor suppressor and mutations in it have been associated with various tumors.

Forme physique

Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable