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SAB4200141

Sigma-Aldrich

Anti-Rictor antibody produced in rabbit

~1-2 μg/mL, affinity isolated antibody

Synonyme(s) :

ANTI-Pianissimo, ANTI-Rapamycin-insensitive companion of mTOR, ANTI-TORC2-specific protein AVO3, Anti-RPTOR independent companion of MTOR, complex 2, AVO3

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.44

Source biologique

rabbit

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

~190 kDa

Espèces réactives

human, rat, mouse

Conditionnement

antibody small pack of 25 μL

Concentration

~1-2 μg/mL

Technique(s)

immunohistochemistry: 5-10 μg/mL using a working concentration of 5-10 ug/mL is recommended using heat-retrieved formalin-fixed, paraffin-embedded rat testis sections and biotin / ExtrAvidin Peroxidase staining system.
immunoprecipitation (IP): 5-10 μg using a working amount of 5-10 μg is recommended using lysates of human HeLa cells.
western blot: 1-2 μg/mL using a working concentration of 1-2 μg/mL is recommended using whole extracts of mouse C2C12 cells.

Numéro d'accès UniProt

Q6R327

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... RICTOR(253260)
mouse ... rictor(78757)
rat ... rictor(310131)

Catégories apparentées

Description générale

Rictor (rapamycin-insensitive companion of mTOR) is a subunit of the mammalian target of rapamycin complex 2 (mTORC2). The mTORC2 complex contains mTOR, mLST8, mSIN1, deptor, protor-1 and rictor. mTOR substrate that regulates cell size.
m-TOR is a conserved kinase that primarily regulates cell growth. Rictor is a rapamycin-insensitive companion of m-TOR and regulates the phosphorylation of Akt/PKB. Rictor also phosphorylates PKCα and actin cytoskeleton .

Spécificité

Anti-Rictor antibody is specific for human, mouse and rat rictor. In immunoblotting, detection of the rictor band (approx. 190 kDa) is specifically inhibited by the immunizing peptide.

Immunogène

The corresponding sequence is identical in mouse and rat rictor.

Application

Anti-Rictor antibody is suitable for use in immunohistochemistry (5-10 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded rat testis sections and biotin/ExtrAvidin Peroxidase staining system). The product may also be used for immunoprecipitation (5-10 μg using lysates of human HeLa cells), and immunoblot (1-2 μg/mL is using whole extracts of mouse C2C12 cells).

Actions biochimiques/physiologiques

Rictor (rapamycin-insensitive companion of mTOR) regulates cell survival and cytoskeletal organization through the regulation of Akt/ protein kinase B (PKB) and protein kinase C alpha (PKCα).

Forme physique

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

mTORC2 activity is elevated in gliomas and promotes growth and cell motility via overexpression of rictor
Masri J, et al.
Cancer research, 67(24), 11712-11720 (2007)
D D Sarbassov et al.
Science (New York, N.Y.), 307(5712), 1098-1101 (2005-02-19)
Deregulation of Akt/protein kinase B (PKB) is implicated in the pathogenesis of cancer and diabetes. Akt/PKB activation requires the phosphorylation of Thr308 in the activation loop by the phosphoinositide-dependent kinase 1 (PDK1) and Ser473 within the carboxyl-terminal hydrophobic motif by
Targeting mTORC2 inhibits colon cancer cell proliferation in vitro and tumor formation in vivo
Roulin D, et al.
Molecular Cancer, 9(1), 57-57 (2010)
D D Sarbassov et al.
Current biology : CB, 14(14), 1296-1302 (2004-07-23)
The mammalian TOR (mTOR) pathway integrates nutrient- and growth factor-derived signals to regulate growth, the process whereby cells accumulate mass and increase in size. mTOR is a large protein kinase and the target of rapamycin, an immunosuppressant that also blocks
Estela Jacinto et al.
Nature cell biology, 6(11), 1122-1128 (2004-10-07)
The target of rapamycin (TOR) is a highly conserved protein kinase and a central controller of cell growth. In budding yeast, TOR is found in structurally and functionally distinct protein complexes: TORC1 and TORC2. A mammalian counterpart of TORC1 (mTORC1)
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