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SAB4200053

Sigma-Aldrich

Anti-DNA polymerase δ p125 antibody, Rat monoclonal

clone PDG 5G1, purified from hybridoma cell culture

Synonyme(s) :

Anti-POLD1, Anti-Polymerase (DNA directed), delta 1, catalytic subunit 125kDa, Monoclonal Anti-DNA polymerase δ p125 antibody produced in rat

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rat

Forme d'anticorps

purified from hybridoma cell culture

Type de produit anticorps

primary antibodies

Clone

PDG 5G1, monoclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~125 kDa

Espèces réactives

human, mouse, rat

Conditionnement

antibody small pack of 25 μL

Technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 0.5-1.0 μg/mL using Jurkat cells extract

Numéro d'accès UniProt

P28340

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... POLD1(5424)

Catégories apparentées

Description générale

Monoclonal Anti-DNA polymerase d p125 (rat IgG2a isotype) is derived from the hybridoma PDG 5G1 produced by the fusion of mouse myeloma cells (P3X63Ag8.653) and splenocytes from rat immunized with human p125. The catalytic subunit p125 (POLD1), which is encoded by the Pold1 gene, is highly conserved among eukaryotes. Pold1 comprises of two primary functional domains: an exonuclease domain near the N-terminus and the subsequent polymerase domain.

Immunogène

human p125

Application

Monoclonal Anti-DNA polymerase δ p125 antibody produced in rat has been used in:
  • immunoblotting
  • immunoprecipitation
  • immunocytochemistry
  • co-immunoprecipitation

Actions biochimiques/physiologiques

The exonuclease domain of the catalytic subunit p125 (POLD1) has the ability to catalyze 3′−5′ exonucleolytic proofreading and the polymerase domain helps to catalyze DNA synthesis. Mutations in this subunit have been implicated in mammalian embryogenesis and tumorigenesis.

Forme physique

Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

DNA Polymerase delta Is Required for Early Mammalian Embryogenesis
Uchimura A, et al.
Testing, 4(1) (2009)
Human DNA polymerase delta is a pentameric holoenzyme with a dimeric p12 subunit
Khandagale P, et al.
Life science alliance, 2(2) (2019)
Eukaryotic DNA polymerases, a growing family
Huubscher U, et al.
Trends in Biochemical Sciences, 25(3), 143-147 (2000)
DNA Polymerase delta Is Required for Early Mammalian Embryogenesis
Uchimura A, et al.
PLoS ONE, 4(1) (2009)
Prashant Khandagale et al.
Life science alliance, 2(2) (2019-03-20)
Human DNA polymerase delta (Polδ), a holoenzyme consisting of p125, p50, p68, and p12 subunits, plays an essential role in DNA replication, repair, and recombination. Herein, using multiple physicochemical and cellular approaches, we found that the p12 protein forms a

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