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Key Documents

SAB2702214

Sigma-Aldrich

Monoclonal Anti-RFP antibody produced in mouse

clone GT1433, affinity isolated antibody

Synonyme(s) :

Mouse Anti-Ref Fluorescent protein

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

100

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

GT1433, monoclonal

Forme

buffered aqueous solution

Poids mol.

27kDa

Concentration

0.98mg/mL

Technique(s)

immunoprecipitation (IP): suitable
western blot: 5000-20000

Isotype

IgG1

Conditions d'expédition

wet ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Description générale

Red fluorescent protein (RFP) is obtained from the Discosoma coral. It can be used as a fluorescent probe for optical microscopy and acts as a biological marker for biomolecule imaging. RFPs exhibit fluorescence emission beyond 57 0nm. They have lower light-scattering, low photo-toxicity and less autofluorescence interference of cells. RFPs are extremely useful for whole body and deep tissue imaging.

Immunogène

Recombinant protein conjugated synthetic peptide encompassing a sequence within the center region of RFP.

Application

Suggested starting dilutions are as follows: IP: 1:100-1:500, WB: 1:5000-1:20000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. Monoclonal Anti-RFP antibody produced in mouse has been used in chromatin immunoprecipitation (ChIP) assay.

Caractéristiques et avantages

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Autres remarques

Purification: Affinity purified by Protein G

Forme physique

Phosphate-buffered saline, no preservative added.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Misregulation of a DDHD Domain-containing Lipase Causes Mitochondrial Dysfunction in Yeast*
Pradeep Kumar Yadav and Ram Rajasekharan
The Journal of Biological Chemistry (2016)
DNA mimics of red fluorescent proteins (RFP) based on G-quadruplex-confined synthetic RFP chromophores
Nucleic Acids Research, 45(18) (2017)
A monomeric red fluorescent protein
Robert E. Campbell
Proceedings of the National Academy of Sciences of the USA (2002)
Guide to Red Fluorescent Proteins and Biosensors for Flow Cytometry
Kiryl D
Methods in Cell Biology (2011)
Pradeep Kumar Yadav et al.
The Journal of biological chemistry, 291(35), 18562-18581 (2016-07-13)
The DDHD domain-containing proteins, which belong to the intracellular phospholipase A1 (iPLA1) family, have been predicted to be involved in phospholipid metabolism, lipid trafficking, membrane turnover, and signaling. Defective cardiolipin (CL), phosphatidylethanolamine, and phosphatidylglycerol remodeling cause Barth syndrome and mitochondrial
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