S8160

Superoxide Dismutase from bovine liver

lyophilized powder, ≥1500 units/mg protein

• Synonyme(s) : SOD, Superoxide: superoxide oxidoreductase
• Numéro CAS: 9054-89-1
• Numéro de classification (Commission des enzymes): 1.15.1.1 (BRENDA, IUBMB)
• Numéro CE : 232-943-0
• Numéro MDL: MFCD00132404
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Forme: lyophilized powder
• Niveau de qualité: 200
• Activité spécifique: ≥1500 units/mg protein
• Poids mol.: 32.5 kDa
• Composition: Protein, ≥70% biuret
• Numéro d'accès UniProt: P00442
• Température de stockage: −20°C
• Informations sur le gène: cow ... SOD1(281495)

Description

Description générale

Superoxide dismutases are a group of low molecular weight metalloproteins present in all aerobic cells of plants, animals and micro-organisms. They provide protection against damaging reactions with the superoxide radical anion (O2-) by catalyzing its disproportionation into oxygen and hydrogen peroxide.

Application

Superoxide dismutase from bovine liver has been used in a study to determine that hypercholesterolemia increases endothelial superoxide anion production. Superoxide dismutase from bovine liver has also been used in a study to investigate diazo coupling, subunit interactions and electrophoretic variants of bovine erythrocyte superoxide dismutase.

Actions biochimiques/physiologiques

Catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. Plays a critical role in the defense of cells against the toxic effects of oxygen radicals. Competes with nitric oxide (NO) for superoxide anion (which reacts with NO to form peroxynitrite), thereby SOD promotes the activity of NO. SOD has also been shown to suppress apoptosis in cultured rat ovarian follicles, neural cell lines, and transgenic mice.

Définition de l'unité

One unit will inhibit reduction of cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25 °C in a 3.0 mL reaction volume. Xanthine oxidase concentration should produce an initial ΔA₅₅₀ of 0.025 ± 0.005 per min.

Forme physique

Lyophilized powder containing potassium phosphate buffer salts

Remarque sur l'analyse

For assay method, see McCord, J.M. and Fridovich, I., J. Biol. Chem., 244, 6049 (1969).

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)