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Principaux documents

RAB1574

Sigma-Aldrich

Human Matriptase ELISA

for serum, plasma and cell culture supernatants

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About This Item

Code UNSPSC :
41116158
Nomenclature NACRES :
NA.32

Espèces réactives

human

Conditionnement

kit of 96 wells (12 strips x 8 wells)

Technique(s)

ELISA: suitable

Entrée

sample type plasma
sample type cell culture supernatant(s)
sample type serum

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 0.41 ng/mL
standard curve range: 0.410-100 ng/mL

Méthode de détection

colorimetric

Conditions d'expédition

wet ice

Température de stockage

−20°C

Informations sur le gène

human ... ST14(6768)

Description générale

The ST14 (suppression of tumorigenicity 14) gene encodes a cell surface serine protease which is also called as matriptase. The ST14 gene is mapped to human chromosome 11q24.3.
This ELISA antibody pair detects Human Matriptase. Other species not yet determined.

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached Protocolfor details.

Actions biochimiques/physiologiques

St14 (suppression of tumorigenicity 14) forms complexes with serine proteases inhibitors HAIs (hepatocyte growth factor activator inhibitors), to prevent its proteolytic activity. ST14 expression is required for epithelial mesenchymal transition in tumor cells. St14′s biological activity is specific to different substrates, which includes extracellular matrix proteins, hepatocyte growth factor, epidermal growth factor receptor (EGFR), adhesion molecules including Mac25 (insulin-like growth factor-binding protein 7) and Tie2 (angiopoietin-1 receptor) , proteases including urokinase plasminogen activator (uPA), prostasin (PRSS8), matrix metalloprotease 3 (MMP3). Upregulation of the gene is found in progressed epithelial cancer but lower expression of the gene is observed in colon cancer and hence it is termed ‘suppression of tumors′.

Autres remarques

A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number.

Pictogrammes

Corrosion

Mention d'avertissement

Warning

Mentions de danger

Conseils de prudence

Classification des risques

Met. Corr. 1

Code de la classe de stockage

8A - Combustible corrosive hazardous materials

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Impact of suppression of tumorigenicity 14 (ST14)/serine protease 14 (Prss14) expression analysis on the prognosis and management of estrogen receptor negative breast cancer.
Kim S, et al.
Oncotarget, 7(23), 34643-34643 (2016)
Autosomal recessive ichthyosis with hypotrichosis caused by a mutation in ST14, encoding type II transmembrane serine protease matriptase.
Basel-Vanagaite L, et al.
American Journal of Human Genetics, 80(3), 467-477 (2007)
Activation of proHGF by St14 induces mouse embryonic stem cell differentiation.
Yan X, et al.
Protein & cell, 7(8), 601-605 (2016)

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