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Key Documents

R9388

Sigma-Aldrich

Retinol Binding Protein from human urine

buffered aqueous solution

Synonyme(s) :

CRBP, Cellular retinol binding protein, RBP, RBP-1, Retinol Binding Protein I

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About This Item

Numéro MDL:
MFCD00165761
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.56

Source biologique

human urine

Niveau de qualité

200

Pureté

≥85% (SDS-GE)

Forme

buffered aqueous solution

Technique(s)

cell culture | embryo: suitable
transfection: suitable

Numéro d'accès UniProt

P09455

Conditions d'expédition

dry ice

Température de stockage

−20°C

Informations sur le gène

human ... RBP1(5947)

Catégories apparentées

Description générale

Retinol Binding Protein (RBP) is a member of lipocalin superfamily. The normal concentration of RBP in serum is 40 to 50 μg/mL and has a molecular weight of 21 kDa. It has a turnover rate of 4 to 24 h. RBP is produced in the liver in response to retinol binding trigger. It is also produced in kidney, sertoli cells of testis. RBP contains a single globular domain and a highly conserved N-terminal region. It has eight antiparallel β sheets in the centre which binds retinol. In human chromosome, the gene encoding RBP is localised at 3q23.

Application

Retinol Binding Protein from human urine has been used:
  • as one of the protein in microinjecting mesencephalic cavity of chicken eggs to monitor embryonic cerebrospinal fluid/embryonic serum protein transfer
  • as an additive in basal medium for RNA transfection experiments
  • in the antibody-derivatized affinity pipettes preparation for the capture of affinity proteins

Actions biochimiques/physiologiques

Inflammation induced by lipopolysaccharide (LPS) exposure caused a reduction in the level of retinol binding protein produced by the liver.
Retinol Binding Protein (RBP) is a specific protein for the transport of vitamin A. The apo-RBP, formed after delivering retinol to the tissues is catabolized by the kidney. The plasma concentration of RBP is less in diabetes mellitus patients. RBP binds to the carrier protein transerythrin, to prevent its loss during glomerular filtration. RBP is implicated in initial neurogenesis and neuroepithelial cell proliferation. RBP is responsible for blepharophimosis syndrome (BPES).

Forme physique

Solution in 10 mM sodium phosphate, pH 7.4, 150 mM NaCl, and 0.05% NaN3

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Consulter la Bibliothèque de documents

Plasma retinol binding protein: structure and function of the prototypic lipocalin
Newcomer ME and Ong DE
Biochimica et Biophysica Acta, Protein Structure and Molecular Enzymology, 1482(1-2), 57-64 (2000)
Assignment of the cellular retinol-binding protein 1 gene (RBP1) and of the coatomer beta' subunit gene (COPB2) to human chromosome band 3q23 by in situ hybridization
De Baere E, et al.
Cytogenetics and Cell Genetics, 82, 226-227 (1998)
Laboratory Tests for the Assessment of Nutritional Status, Second Edition (1999)
Vitamin A in Health and Disease, 87-95 (1994)
F J Rosales et al.
Journal of lipid research, 37(5), 962-971 (1996-05-01)
The acute inflammatory response to tissue injury and infection is associated with low concentrations of plasma retinol and its specific transport proteins, retinol-binding protein (RBP) and transthyretin (TTR). To examine the kinetics and mechanism of hyporetinemia, we have induced acute
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