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R7256

Sigma-Aldrich

RPMI 1640 Vitamins Solution (100×)

liquid, sterile-filtered, BioReagent, suitable for cell culture

Synonyme(s) :

RPMI 1640 Solution, RPMI 1640 Vitamins, Vitamins Solution

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About This Item

Code UNSPSC :
12352205
Nomenclature NACRES :
NA.75

Stérilité

sterile-filtered

Gamme de produits

BioReagent

Forme

liquid

Technique(s)

cell culture | mammalian: suitable

Impuretés

endotoxin, tested

Couleur

yellow to dark yellow

pH

7.2-7.6

Conditions d'expédition

dry ice

Température de stockage

−20°C

Description générale

Roswell Park Memorial Institute medium is commonly referred as RPMI. It is a form of medium used in cell culture and tissue culture. This medium contains phosphate and is formulated for use in a 5% carbon dioxide atmosphere. RPMI 1640 is used for the serum-free expansion of human lymphoid cells.

Application

RPMI 1640 Vitamins Solution (100×) has been used:
  • in the preparation of CeM1 medium for the isolation of Caenorhabditis elegans germ cells
  • as a component of the amino-acid-deprived medium for the starvation of intestinal porcine enterocytes
  • as a component of the stage-8 medium for culturing of human pluripotent stem cell-derived 3D pancreatic progenitor clusters
RPMI 1640 Vitamins Solution (100×) is used as a supplement in cell culture media.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Leucine stimulates ASCT2 amino acid transporter expression in porcine jejunal epithelial cell line (IPEC-J2) through PI3K/Akt/mTOR and ERK signaling pathways
Shihai Z, et al.
Amino Acids, 46(12) (2014)
Indranil Biswas et al.
Journal of bacteriology, 189(18), 6521-6531 (2007-07-10)
Streptococcus mutans, the principal causative agent of dental caries, produces four glucan-binding proteins (Gbp) that play major roles in bacterial adherence and pathogenesis. One of these proteins, GbpC, is an important cell surface protein involved in biofilm formation. GbpC is
Simon Kemmerling et al.
Journal of structural biology, 177(1), 128-134 (2011-11-19)
A versatile methodology for electron microscopy (EM) grid preparation enabling total content sample analysis is presented. A microfluidic-dialysis conditioning module to desalt or mix samples with negative stain solution is used, combined with a robotic writing table to micro-pattern the
Simon Kemmerling et al.
Journal of structural biology, 183(3), 467-473 (2013-07-03)
The stochastic nature of biological systems makes the study of individual cells a necessity in systems biology. Yet, handling and disruption of single cells and the analysis of the relatively low concentrations of their protein components still challenges available techniques.
Alexandra S Vagasi et al.
Bio-protocol, 7(15) (2017-09-05)
The Caenorhabditis elegans germ line is an important model system for the study of germ stem cells. Wild-type C. elegans germ cells are syncytial and therefore cannot be isolated in in vitro cultures. In contrast, the germ cells from tumorous
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