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Principaux documents

R6148

Sigma-Aldrich

RNase A solution

Synonyme(s) :

GenElute RNAse A

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About This Item

Code UNSPSC :
12352204
Nomenclature NACRES :
NA.52

Source biologique

bovine pancreas

Niveau de qualité

Forme

liquid

Activité spécifique

1500 units/mL
3000 units/mL

Concentration

20 mg/mL in Tris buffer

Technique(s)

DNA purification: suitable

Température de stockage

room temp

Informations sur le gène

Description générale

Ribonuclease A (RNase A), a protein composed of three α helices and seven β strands arranged in two "lobes” holds significance as the prototypical domain-swapped protein. It is known for its diverse oligomeric states. The RNase A family derives its name from bovine pancreatic RNase A and is specific to vertebrates. In humans, this family encompasses eight functional members, showcasing its biological relevance.
For sample preparation in GenElute kits.

Application

RNase A solution has been used:

  • in apoptosis assay of Hep-2 cells
  • for the colorimetric quantification of methylated DNA
  • for DNA and RNA extraction
  • in DNA laddering analysis
  • in cell cycle analysis
  • as a component in phosphate-buffered saline (PBS) to incubate cells for flow cytometry

Actions biochimiques/physiologiques

Ribonuclease A or bovine pancreatic ribonuclease A (RNase A) enzyme is an endoribonuclease that displays a specific ability to cleave single-stranded RNA on the 3′ side of pyrimidine residues. This endonuclease with 124 amino acids plays a key role in gene duplication, cellular metabolism, and cancer therapy. Its overexpression is closely associated with several types of cancer. Hence, RNase A has the potential to be a promising new biomarker for the diagnosis of malignant tumors and is also used as a specific target for new drug discovery. RNase A is commonly used for the isolation of plasmid DNA.

Notes préparatoires

For usage requirements, please refer to the kit procedure.

Informations légales

GenElute is a trademark of Sigma-Aldrich Co. LLC

Préparation d'échantillons

Réf. du produit
Description
Tarif

Pictogrammes

Health hazard

Mention d'avertissement

Danger

Mentions de danger

Conseils de prudence

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Consulter la Bibliothèque de documents

Determination of the presence of 5-methylcytosine in Paramecium tetraurelia
Singh A, et al.
PloS one, 13(10), e0206667-e0206667 (2018)
Abdou ElSharawy et al.
Oncotarget, 7(46), 75353-75365 (2016-09-30)
The emerging potential of miRNAs as biomarkers for cancer detection demands parallel evaluation of strategies for reliable identification of disease-related signatures from easily accessible and pertinent body compartments. Here, we addressed whether efficient concentration of circulating miRNA-carrying particles is a
Phenotypic plasticity and biogeographic variation in physiology of habitat-forming seaweed: response to temperature and nitrate
Flukes EB, et al.
Journal of Phycology, 51(5), 896-909 (2015)
Lenka Senohrabkova et al.
Current genetics, 65(4), 919-940 (2019-02-05)
Cells have elaborated a complex strategy to maintain protein homeostasis under physiological as well as stress conditions with the aim to ensure the smooth functioning of vital processes and producing healthy offspring. Impairment of one of the most important processes
Shiwen Zhang et al.
International journal of nanomedicine, 9, 1931-1946 (2014-05-03)
Gold nanorods (AuNRs) have been used in plasmonic photothermal therapy (PPTT), which is thought to be more efficient and selective than conventional photothermal therapy. The efficiency and safety of PPTT can be improved by functionally modifying the gold nanorods with

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