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R4654

Sigma-Aldrich

Anti-Rab5 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonyme(s) :

Anti-Ras-Related Protein Rab5A

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About This Item

Numéro MDL:
MFCD01322589
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

rabbit

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~24 kDa

Espèces réactives

human, mouse, rat

Concentration

~1 mg/mL

Technique(s)

indirect immunofluorescence: 5-10 μg/mL using rat NRK and mouse NIH-3T3 cells.
western blot: 2.5-5 μg/mL using whole extract of human A431 cells

Numéro d'accès UniProt

P20339

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... RAB5A(5868)
mouse ... Rab5a(271457)
rat ... Rab5a(64633)

Catégories apparentées

Description générale

Ras related protein consists of three isoforms such as Rab5a, Rab5b and Rab5c. Rab5a is encoded by the gene mapped to human chromosome 3p24.3. This gene codes for 23.66 kDa Rab5a protein.

Immunogène

synthetic peptide corresponding to amino acid residues 176-188 of human Rab5 with C-terminal added cysteine, conjugated to KLH.

Application

Anti-Rab5 antibody produced in rabbit has been used in western blotting and immunocytochemistry.

Actions biochimiques/physiologiques

Rab proteins are members of small GTPases control the exocytosis and endocytosis and regulate vesicular transport. Rab5 regulates endocytic pathway in mammalian cells and an activator of autophagy. It mediates macroautophagy of cells expressing aggregates of mutant Huntingtin protein.
Rab5a is essential for epidermal growth factor (EGF)-mediated EGF receptor degradation. Overexpression of Rab5a is associated with the development of variety of cancers such as breast cancer, lung cancer, hepatocellular carcinoma (HCC) and ovarian cancer.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

M Guescini et al.
Experimental cell research, 318(5), 603-613 (2012-01-24)
Recent evidence shows that cells exchange collections of signals via microvesicles (MVs) and tunneling nano-tubes (TNTs). In this paper we have investigated whether in cell cultures GPCRs can be transferred by means of MVs and TNTs from a source cell
Brinda Ravikumar et al.
Journal of cell science, 121(Pt 10), 1649-1660 (2008-04-24)
Huntington disease (HD) is caused by a polyglutamine-expansion mutation in huntingtin (HTT) that makes the protein toxic and aggregate-prone. The subcellular localisation of huntingtin and many of its interactors suggest a role in endocytosis, and recently it has been shown
Regulated dynamic trafficking of neurexins inside and outside of synaptic terminals
Neupert C, et al.
The Journal of Neuroscience, 35(40), 13629-13647 (2015)
Site-specific monoubiquitination downregulates Rab5 by disrupting effector binding and guanine nucleotide conversion
Shin D, et al.
eLife, 6, e29154-e29154 (2017)
Rab5a promotes the migration and invasion of hepatocellular carcinoma by up-regulating Cdc42
Yang X, et al.
International Journal of Clinical and Experimental Pathology, 11(1), 224-231 (2018)
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