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R4533

Sigma-Aldrich

RNAzol® RT

For processing total and small RNA from human, animal, plant, bacterial,and viral samples

Synonyme(s) :

single step RNA isolation, single step RNA purification, total RNA isolation, total RNA purification

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.52

Niveau de qualité

100

Utilisation

 mL sufficient for 107 cells
 mL sufficient for 100 mg tissue (or)

Catégories apparentées

Description générale

RNAzol RT is a quick and convenient reagent for use in the single-step isolation of total and small RNA from biological samples of human, animal, plant, yeast, bacterial, and viral origin. A convenient single-step liquid phase separation results in the isolation of RNA from DNA, protein, polysaccharides, and other molecules. RNAzol® RT can be used to isolate separate fractions of mRNA and micro RNA or to isolate total RNA, containing all classes of RNA in a single fraction.

This product, a mixture of guanidine thiocyanate and phenol in a monophase solution, effectively dissolves DNA, RNA, and protein on homogenization or lysis of tissue sample. Addition of water to the mixture allows for the precipitation of DNA, proteins, polysaccharides and other molecules, which can then be removed by centrifugation. RNA can then be isolated by alcohol precipitation, washing and solubilization. Chloroform-induced phase separation is not necessary. One mL of RNAzol® RT is sufficient to isolate RNA from up to 100 mg of tissue or 10e7 cells.

This is one of the most effective methods for isolating total and small RNA and can be completed at room temperature in less than 1 hour starting with fresh tissue or cells. The procedure is very effective for isolating RNA molecules of all types: large nuclear RNA, rRNA, mRNA, small RNA and micro RNA. The resulting RNA is intact with little or no contaminating DNA and protein.

Application

Isolated RNA can be used for Northern blots, RNase protection assay, microarrays, PCR, and other molecular biology applications.
RNAzol RT is a highly effective reagent for the single-step isolation of total and small RNA from human, animal, plant, bacterial and viral origin samples. The RNA isolation method based on this reagent is performed at room temperature, without the use of chloroform for phase separation, and allows for use in RT-PCR without requiring DNase treatment.

Caractéristiques et avantages

  • Isolates micro RNA and total RNA isolation of pure and undegraded RNA from biological samples
  • High yields and increased quality of isolated RNA
  • Completed at room temperature in less than an hour starting with fresh tissue or cells

Autres remarques

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Informations légales

RNAZol is a registered trademark of Molecular Research Center, Inc.

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2

Code de la classe de stockage

8A - Combustible, corrosive hazardous materials

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

230.0 °F

Point d'éclair (°C)

110 °C

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Paulina Andryka-Dudek et al.
International journal of molecular sciences, 20(5) (2019-03-01)
Short-term (3 h) treatment of embryos isolated from dormant apple (Malus domestica Borkh.) seeds with NO donors stimulates their transition from dormancy to germination. Seed dormancy is maintained by ABA, while germination is controlled mainly by gibberellins (GAs) and jasmonic
Arturo Vargas et al.
Zebrafish, 15(4), 404-419 (2018-03-29)
A desirable goal of the aquaculture sector is to replace most of fish meal and fish oil with more sustainable, cost-effective, and environmental friendly ingredients ensuring fish health and welfare standards. Due to minimal environmental impact, compared with most conventional
Andrea Miccoli et al.
Marine environmental research, 127, 137-147 (2017-04-17)
Natural/synthetic Endocrine Disrupting Chemicals (EDCs) may display estrogenic activity and a lower potency than 17β-estradiol. Nonetheless, their concentrations and additive effects can affect the endocrine system and reproductive processes related to the Hypothalamic-Pituitary-Gonadal (HPG) axis. Because of their persistence in
The mycotoxin deoxynivalenol predisposes for the development of Clostridium perfringens-induced necrotic enteritis in broiler chickens.
Antonissen G
PLoS ONE, 9(9), e109775-e109775 (2014)
Comparison of Two Available RNA Extraction Protocols for microRNA Amplification in Serum Samples.
Bergallo M
Journal of Clinical Laboratory Analysis, 30(4), 277-283 (2016)
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