Accéder au contenu
MilliporeSigma
Toutes les photos(1)

Documents

R1137

Sigma-Aldrich

Hind III from Haemophilus influenzae

Restriction Enzyme

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(1)

About This Item

Numéro CAS:
81295-22-9
Numéro de classification (Commission des enzymes):
3.1.21.4 (BRENDA, IUBMB)
Numéro MDL:
MFCD00166570
Code UNSPSC :
12352204

Qualité

for molecular biology

Forme

buffered aqueous glycerol solution

Concentration

≥10000 units/mL
10,000 units/mL

Conditions d'expédition

wet ice

Température de stockage

−20°C

Vous recherchez des produits similaires ? Visite Guide de comparaison des produits

Spécificité

Recognition sequence: 5′-A/AGCTT-3′
Cutting results: 2-10-fold Hind III overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: 65 °C for 15 minutes

Application

HindIII, a restriction endonuclease, is used in molecular biology applications to cleave DNA at the recognition site 5′-A/AGCTT-3′ to generate DNA fragments with cohesive 5′-ends.

Autres remarques

Supplied with 10x Restriction Enzyme Buffer SB (B8781)
Comment: Hind III under suboptimal reaction conditions will cleave secondary recognition sites (star activity).

Forme physique

Solution in 10 mM Tris-HCl, pH 7.5, 0.1 mM EDTA, 1 mM dithioerythritol, 250 mM NaCl, 0.01% polydocanol (v/v), 50% glycerol (v/v) at 4 °C

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Stephen J King et al.
Molecular biology of the cell, 14(12), 5089-5097 (2003-10-21)
Cytoplasmic dynein and dynactin are megadalton-sized multisubunit molecules that function together as a cytoskeletal motor. In the present study, we explore the mechanism of dynein-dynactin binding in vitro and then extend our findings to an in vivo context. Solution binding
M Hsu et al.
Biochemistry, 17(1), 131-138 (1978-01-10)
In the presence of 100 mM Tris buffer (pH 7.5) and 1-10 mM Mg2+ EcoRI endonuclease cleaves DNA at a specific nucleotide sequence and in a characteristic way: -GAATTC-. But if Mg2+ is replaced by Mn2+, the specificity of the
Tomás Brdicka et al.
The Journal of experimental medicine, 196(12), 1617-1626 (2002-12-18)
A key molecule necessary for activation of T lymphocytes through their antigen-specific T cell receptor (TCR) is the transmembrane adaptor protein LAT (linker for activation of T cells). Upon TCR engagement, LAT becomes rapidly tyrosine phosphorylated and then serves as
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according
Cong Zhu et al.
Nucleic acids research, 41(4), 2455-2465 (2013-01-11)
Zinc-finger nucleases (ZFNs) have been used for genome engineering in a wide variety of organisms; however, it remains challenging to design effective ZFNs for many genomic sequences using publicly available zinc-finger modules. This limitation is in part because of potential
Afficher tous les articles scientifiques apparentés

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique