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Key Documents

P8999

Sigma-Aldrich

Anti-p53 antibody, Mouse monoclonal

clone DO-7, purified from hybridoma cell culture

Synonyme(s) :

Anti-TRP53, Anti-Transformation-related protein 53

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About This Item

Numéro MDL:
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

Conjugué

unconjugated

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

DO-7, monoclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~53 kDa

Espèces réactives

human

Concentration

~2 mg/mL

Technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 0.1-0.2 μg/mL using human A431

Isotype

IgG2b

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... TP53(7157)

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Description générale

Monoclonal anti-p53, (mouse IgG2b isotype) is derived from the hybridoma DO-7 produced by the fusion of mouse myeloma cells (SP2 cells) and splenocytes from BALB/c mice immunized with recombinant human wild type p53. The p53 gene is located on chromosome 17p. The p53 protein is highly conserved and expressed in normal tissues.

Application

Monoclonal Anti-p53 antibody produced in mouse has been used in:
  • immunoblotting
  • enzyme linked immunosorbent assay (ELISA)
  • immunocytochemistry
  • immunohistochemistry
  • immunoprecipitation

Actions biochimiques/physiologiques

Elevation of p53 protein induces the transcriptional activation of multiple genes, including p21waf1. p21waf1 (cyclin-dependent kinase inhibitor 1A) interacts directly with cyclin dependent kinases, important for cell cycle progression, thereby inhibiting their activity and resulting in cell cycle arrest.
p53 is a transcription factor that regulates several critical cellular functions such as DNA repair, apoptosis, and cell cycle arrest. p53 primarily functions as a tumor suppressor and inactivates apoptosis inhibitors such as survivin, MCL-1 and BCL-2. Mutations in p53 gene occur most commonly in a wide range of human cancers. Thus, analysis of p53 function has important implications in cancer pathogenesis and therapy . Monoclonal Anti-p53 antibody specifically reacts with p53 in humans.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

DNA damage-induced activation of p53 by the checkpoint kinase Chk2
Hirao A, et al.
Science (New York, N.Y.), 287(5459), 1824-1827 (2000)
New Insights into p53 Signaling and Cancer Cell Response to DNA Damage: Implications for Cancer Therapy.
Mirzayans, R., et al.
Journal of Biomedicine and Biotechnology, doi: 10-doi: 10 (2012)
Role of p53, apoptosis, and cell proliferation in early stage Epstein-Barr virus positive and negative gastric carcinomas
Ishii HH, et al.
Journal of Clinical Pathology, 57(12), 1306-1311 (2004)
Chk2/hCds1 functions as a DNA damage checkpoint in G1 by stabilizing p53
Chehab NH, et al.
Genes & Development, 14(3), 278-288 (2000)
Predictors of progression in Barrett's esophagus II: baseline 17p (p53) loss of heterozygosity identifies a patient subset at increased risk for neoplastic progression
Reid BJ, et al.
The American Journal of Gastroenterology, 96(10), 2839-2839 (2001)

Articles

p53 regulates gene expression, cell cycle control and functions as a tumor suppressor. Inactivation of p53 is closely tied to cancer development.

Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.

Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.

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