P8953

Platelet-Derived Growth Factor from porcine platelets

PDGF, lyophilized powder, suitable for cell culture

• Synonyme(s) : PDGF
• Numéro MDL: MFCD00166903
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.77

Propriétés

• Source biologique: Porcine
• Niveau de qualité: 200
• Pureté: ≥97% (SDS-PAGE and N-terminal analysis)
• Forme: lyophilized powder
• Puissance: 39.00 ng/mL EC₅₀
• Qualité: endotoxin tested
• Poids mol.: glycoprotein 38 kDa
• Conditionnement: pkg of 5 μg
• Conditions de stockage: avoid repeated freeze/thaw cycles
• Technique(s): cell culture | mammalian: suitable
• Impuretés: ≤1 EU/μg
• Couleur: white
• Numéro d'accès UniProt: P20034
• Température de stockage: −20°C
• Informations sur le gène: pig ... PDGFB(100626465)

Description

Application

PDGF elicits multifunctional actions with a variety of cells, including mitogenesis of mesoderm-derived cells, increased extracellular matrix synthesis, and chemotaxis and activation of neutrophils, monocytes and fibroblasts.

Actions biochimiques/physiologiques

The principal mitogen found in mammalian serum, released from platelets during clot formation. Elicits mitogenesis of mesoderm-derived cells, increased extracellular matrix synthesis, and chemotaxis and activation of neutrophils, monocytes and fibroblasts. Mitogenic for dermal and tendon fibroblasts, vascular smooth muscle cells, glial cells and chondrocytes. Appears to interact with transforming growth factor-β1 in accelerating wound healing. However, platelet-derived growth factor (PDGF) may also be pathogenic in arteriosclerosis and neoplasia.

Forme physique

Lyophilized from a 0.2 μm filtered solution in 40% acetonitrile and 0.1% trifluoroacetic acid, pH 2.0.

Remarque sur l'analyse

The mitogenic activity is tested in culture using Swiss 3T3 cells or NR6-3T3 fibroblasts.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)