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P7937

Sigma-Aldrich

Protein Phosphatase-1 Catalytic Subunit, α-Isoform from rabbit

5,000-15,000 units/mg protein, recombinant, expressed in E. coli, lyophilized powder

Synonyme(s) :

Protein phosphatase

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About This Item

Numéro de classification (Commission des enzymes):
3.1.3.16 (BRENDA, IUBMB)
Numéro MDL:
MFCD01635658
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32

Source biologique

rabbit

Niveau de qualité

200

Produit recombinant

expressed in E. coli

Pureté

≥80% (GE)

Forme

lyophilized powder

Activité spécifique

5,000-15,000 units/mg protein

Poids mol.

37.5 kDa

Technique(s)

inhibition assay: suitable

Numéro d'accès UniProt

P62139

Température de stockage

−20°C

Informations sur le gène

rabbit ... PPP1CA(100009298)

Description générale

Protein phosphatase1 (PP1) is a heterodimeric enzyme with serine/threonine phosphatase activity. It comprises a catalytic subunit and a targeting subunit or a specific protein inhibitor. The targeting subunit determines the enzyme localization, e.g., to glycogen particles (subunit G) or to myofibrils (subunit M).

Application

Protein Phosphatase-1 Catalytic Subunit, α-Isoform from rabbit has been used as a standard in protein phosphatase-1 inhibition assay.
Protein Phosphatase-1 Catalytic Subunit, α-Isoform from rabbit has been used in protein phosphatase reaction and in the digestion of adipocyte extracts.

Actions biochimiques/physiologiques

Protein phosphatase1 (PP1) is involved in glycogen metabolism and in regulation of muscle contractility. In addition, it is implicated in cell cycle and transcriptional regulation. Recombinant rabbit PP1α has properties in common with the native rabbit muscle protein including size, requirement for Mn2+ for activity, specificity for phosphorylase A, and inhibition by okadaic acid, microcystin LR, and phosphatase inhibitor2 (I-2).

Conditionnement

Package size based on protein content.

Définition de l'unité

One unit will hydrolyze 1 nmole of p-nitrophenyl phosphate per min at pH 7.4 at 30 °C.

Forme physique

Lyophilized powder containing imidazole buffer, pH 7.4, NaCl, DTT, EDTA, MnCl2, Tween 20, and trehalose as stabilizer.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Regulation of protein phosphatase-1
James B Aggen
Chemistry and Biology, 7.1, R13-R23 (2000)
Activity of membrane-associated sucrose synthase is regulated
by its phosphorylation status in cultured cells of sycamore (Acer
pseudoplatanus)
Javier P
Physiologia Plantarum (2004)
Protein kinases and phosphatases: the yin and yang of protein phosphorylation and signaling.
T Hunter
Cell, 80(2), 225-236 (1995-01-27)
M C Faux et al.
Trends in biochemical sciences, 21(8), 312-315 (1996-08-01)
One important regulatory mechanism in the control of phosphorylation events is the subcellular location of phosphatases of kinases. Several serine/threonine phosphatases and kinases have now been shown to be associated with targeting subunits; these contribute to the organization and specificity
Insulin resistance of glycogen synthase mediated by o-linked N-acetylglucosamine.
Parker GJ
The Journal of Biological Chemistry, 278(12), 10022-10027 (2003)
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