P7623

PKD2 Active human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE), aqueous glycerol solution

• Synonyme(s) : Protein Kinase D2
• Numéro MDL: MFCD07371065
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.32

Propriétés

• Produit recombinant: expressed in E. coli
• Niveau de qualité: 200
• Pureté: ≥70% (SDS-PAGE)
• Forme: aqueous glycerol solution
• Activité spécifique: 100 units/mg protein (Bradford)
• Poids mol.: protein apparent mol wt ~140 kDa
• Numéro d'accès UniProt: Q9BZL6
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... PRKD2(25865)

Description

Application

Kinase activity is measured as the molar amount of phosphate incorporated into the CREBTIDE substrate peptide (KRREILSRRPSYR), per minute per mg protein at 30 °C using a final concentration of 50 μM [³²P] ATP.

Actions biochimiques/physiologiques

PKD2 is a novel phorbol ester- and growth factor-stimulated serine/threonine kinase that contains two cysteine-rich motifs at the N terminus, a pleckstrin homology domain, and a catalytic domain. The PKD family of enzymes have been implicated in very diverse cellular functions, including Golgi organization and plasma membrane directed transport, metastasis, immune responses, apoptosis and cell proliferation.

Définition de l'unité

One unit will incorporate one nanomole of phosphate into the CREBTIDE substrate (KRREILSRRPSYR) per minute at 30 °C at pH 7.2 using a final concentration of 50 μM [32P] ATP.

Forme physique

Solution of 50 μl in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM dithiothreitol (DTT), 0.1 mM EGTA, and 30% glycerol.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids