P7607
Monoclonal Anti-Protein Phosphatase 1α antibody produced in mouse
clone PPI-377, ascites fluid
Synonyme(s) :
Anti-PP1α
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About This Item
Produits recommandés
Source biologique
mouse
Conjugué
unconjugated
Forme d'anticorps
ascites fluid
Type de produit anticorps
primary antibodies
Clone
PPI-377, monoclonal
Poids mol.
antigen 37.5 kDa
Contient
15 mM sodium azide
Espèces réactives
rabbit, rat, bovine, human, mouse, monkey
Technique(s)
immunocytochemistry: suitable
microarray: suitable
western blot: 1:500 using mouse fibroblasts cell extract
Isotype
IgG2b
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... PPP1CA(5499)
mouse ... Ppp1ca(19045)
rat ... Ppp1ca(24668)
Description générale
Among the post-translational modifications, phosphorylation is a vital regulatory mechanism of key proteins involved in specific pathways. Reverse phosphorylation has become recognized as the key process of regulation of gene expression, cellular proliferation, differentiation in Eukaryotes. Protein phosphatases, like kinases, are a class of enzymes that regulate protein phosphorylation. The serine/threonine phosphatases have been classified into four groups which include PP1, PP2A, PP2B (also termed calcineurin) and PP2C on the basis of differences in their biochemical properties. PP1 catalyzes a wide range of protein dephosphorylation reactions in a tightly regulated manner and is expressed abundantly in the brain. PP1 has broad functions covering glycogen metabolism, protein synthesis, cell cycle and growth and muscle contractility. PP1 forms exclusive complexes with >50 regulatory subunits that allows for restricted subcellular location and thereby distinct cellular functions. There are 3 isoforms of PP1 (α, β and γ1) with 90% homology. PP1α is expressed in brain, specifically in cerebellum, prefrontal cortex and synapses
Monoclonal Anti-Protein Phosphatase 1α specifically recognizes an epitope within the catalytic subunit of PP1α isoform (37.5 kDa).
Monoclonal Anti-Protein Phosphatase 1α specifically recognizes an epitope within the catalytic subunit of PP1α isoform (37.5 kDa).
Spécificité
The antibody reacts with protein phosphatase 1α and recognizes an epitope within its catalytic subunit.
Immunogène
recombinant rabbit protein phosphatase 1α (PP1α) catalytic subunit
Application
Anti-Protein Phosphatase 1α is suitable for immunoblotting at a working dilution of 1:500 using mouse fibroblasts cell extract. A dilution of 1:200 has been used for immunoblotting in myocardial protein extracts of Göttinger minipigs. For immunoprecipitation, 1 μg antibody/20 μg of pig protein extracts was suitable. The antibody is also suitable for immunocytochemistry and protein microarray.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
nwg
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Jill R Bordelon et al.
Cerebral cortex (New York, N.Y. : 1991), 15(12), 1928-1937 (2005-03-11)
Prefrontal cortical functioning depends on D1 family receptors and their complex signal transduction cascade, including protein phosphatase-1 (PP1). Three PP1 isoforms are prominent in the brain: PP1alpha, PP1beta and PP1gamma1. PP1 localization by a variety of scaffolding proteins is critical
Differential cellular and subcellular localization of protein phosphatase 1 isoforms in brain
Strack S et al
The Journal of Comparative Neurology, 25, 373-384 (1999)
Yang Jiao et al.
Acta pharmacologica Sinica, 28(12), 1957-1967 (2007-11-23)
HMGB1 (high-mobility group box-1) is a nuclear protein containing a consensus RB (retinoblastoma)-binding LXCXE motif. In this study, we studied the potential association of HMGB1 and RB and the in vitro and in vivo activities of HMGB1 in human breast
Andreas Totzeck et al.
American journal of physiology. Heart and circulatory physiology, 295(5), H2106-H2112 (2008-10-07)
Cardiac connexin 43 (Cx43) is involved in infarct propagation, and the uncoupling of Cx43-formed channels reduces infarct size. Cx43-formed channels open upon Cx43 dephosphorylation, and ischemic preconditioning (IP) prevents the ischemia-induced Cx43 dephosphorylation. In addition to the sarcolemma, Cx43 is
A Fischer et al.
Toxicology and applied pharmacology, 245(1), 9-20 (2010-02-23)
Cellular uptake of microcystins (MCs), a family of cyclic cyanobacterial heptapeptide toxins, occurs via specific organic anion transporting polypeptides (OATPs), where MCs inhibit serine/threonine-specific protein phosphatase (PP). Despite comparable PP-inhibitory capacity, MCs differ greatly in their acute toxicity, thus raising
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