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P6097

Sigma-Aldrich

Precast Agarose Gels

24 wells (2 × 12-well portrait orientation)

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About This Item

Code UNSPSC :
41105300

Qualité

for molecular biology

Niveau de qualité

Forme

gel form

Composition

(Precast gels contain 4.0% agarose in 1X TBE buffer with 0.5 μg/ml ethidium bromide.DNA agarose gels contain 1× TBE buffer (0.089 M Tris-borate, pH 8.3, 2 mM EDTA) and 0.5 mg/ml ethidium bromide. RNA agarose gels contain 1× MOPS (20 mM MOPS, 5 mM sodium acetate, 1 mM EDTA and 1 mM EGTA, pH 7.0) Quality)

Température de stockage

room temp

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Description générale

Precast agarose gels are designed for high resolution separation of nucleic acids, producing sharp DNA bands with low background fluorescence. Precast gels are individually foil sealed in plastic trays and are supplied in a box of twenty.

Gel size: 6.0 × 9.5 cm, 5.5 mm thick
Well orientation: 24-well, arranged as a 2 x 12 well portrait style

Application

Precast agarose gels are designed for high resolution separation of nucleic acids, producing sharp DNA and RNA bands with low background fluorescence. Precast gels are individually foil sealed in plastic trays that help eliminate contamination from equipment and handling. Gels can be electrophoresed right in the plastic tray.

TBE - agarose gels are available in two common concentrations (1% and 4%) for DNA electrophoresis. Ethidium bromide (0.5 μg/ml) is included in the TBE-agarose gels for easy visualization. TBE-agarose gels are available in 8, 20 and 24 well formats. The separation range for the 1.0% gel is 500-1000 bp and 8-1000 bp for the 4% gel.

MOPS - agarose gels (1.25%) are available without denaturants (e.g. formaldehyde or glyoxal) for the analysis of total RNA, in vitro RNA transcripts, and Northern blotting. RNA electrophoresis usually does not interfere with staining. If significant secondary structure is suspected, the RNA should be denatured by a method listed in the product insert. The separation range for the 1.25% MOPS gel is 0.25-10 kb.
Suitable for separation of DNA fragments ranging from 8 – 1000 base pairs.

Conditionnement

Package of 20 individually foil-wrapped gels.

Composants

Precast gels contain 4.0% agarose in 1X TBE buffer with 0.5 μg/ml ethidium bromide.

Autres remarques

DNA agarose gels contain 1× TBE buffer (0.089 M Tris-borate, pH 8.3, 2 mM EDTA) and 0.5 mg/ml ethidium bromide.
RNA agarose gels contain 1× MOPS (20 mM MOPS, 5 mM sodium acetate, 1 mM EDTA and 1 mM EGTA, pH 7.0)

Qualité

No detected DNase or RNase activity

Forme physique

Gel size: 6.0 × 9.5 cm, 5.5 mm thick
Cassette size: 6.8 × 10.2 cm container tray; fits common horizontal electrophoresis units
Sample format: 8-well (portrait), 20-well (landscape), 24-well (2 × 12 well portrait)

Code de la classe de stockage

13 - Non Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)


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