P4874

Anti-PADI4 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonyme(s) : Anti-PAD4, Anti-PADI5, Anti-PDI4, Anti-Peptidyl Arginine Deiminase Type IV, Anti-Peptidyl Arginine Deiminase V
• Numéro MDL: MFCD09039201
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Conjugué: unconjugated
• Forme d'anticorps: IgG fraction of antiserum
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen 67 kDa
• Espèces réactives: human
• Technique(s): immunoprecipitation (IP): 2-4 μL using DMSO-treated HL60 cells; western blot: 1:500-1:1,000 using DMSO-treated HL60 cells
• Numéro d'accès UniProt: Q9UM07
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... PADI4(23569)

Description

Description générale

PAD4 (also designated PADI4 or PAD type IV), one of the members of the peptidyl arginine deiminases (PADIs).

Peptidyl arginine deiminase 4 (PADI4) protein consists of 663 amino acids. PADI4 gene is localized to human chromosome 1p36. It is expressed in hematopoietic tissues, such as spleen, thymus, peripheral blood leucocytes, fetal liver and bone marrow.

Immunogène

synthetic peptide corresponding to amino acids 91-107 of human PADI4, conjugated to KLH via a C-terminal added cysteine residue. The immunizing peptide is specific to human PADI4.

Application

Anti-PADI4 (N-terminal) antibody produced in rabbit has been used in immunoblotting,immunoprecipitation and immunofluorescence.

Actions biochimiques/physiologiques

PAD4 is recruited to the pS2 promoter (an estrogen responsive gene) following hormone induction when the gene is transcriptionally downregulated.

Peptidyl arginine deiminase 4 (PADI4) is responsible for the post-translational conversion of peptidylarginine to citrulline, in the presence of calcium ions. In individuals with rheumatoid arthritis (RA), this gene is expressed in hematological cells and synovial tissues, and variant in this gene is linked with susceptibility to RA. The expression of this protein is linked with DNA hypermethylation in acute promyelocytic leukemia (APL).

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable