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Key Documents

P4505

Sigma-Aldrich

Protamine sulfate salt from herring

Grade III, histone, free(Millon test)

Synonyme(s) :

Clupeine

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About This Item

Numéro CAS:
9007-31-2
Numéro MDL:
MFCD00132091
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.77

Source biologique

fish (herring)

Niveau de qualité

200

Type

Grade III

Forme

powder

Impuretés

histone, free (Millon test)

Solubilité

6 M HCl: 25 mg/mL, clear, colorless to very faintly yellow

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Application

May be used for the removal of DNA from protein samples or for the purification of DNA binding proteins. It may be used as an alternative to polybrene in retroviral-mediated gene transfer.

Actions biochimiques/physiologiques

Protamine in the form of solid lipid nanoparticles (SLN) promoted transfection with plasmid DNA more efficiently and with less cytotoxicity than comparable SLNs composed of Esterquat-1.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

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I Saiki et al.
Japanese journal of cancer research : Gann, 81(6-7), 668-675 (1990-06-01)
We have investigated the anti-angiogenic effect of a polymeric peptide based on the Arg-Gly-Asp (RGD) core sequence of fibronectin as a monomer unit, i.e., poly(RGD), in syngeneic mice and in vitro. Single intratumoral administration of poly(RGD) on day 0, 1
C Toniolo et al.
Biochimica et biophysica acta, 576(2), 429-439 (1979-02-26)
The three main components YI, YII, and Z of clupeine, a protamine from herring, have been purified and characterized. The conformational preferences of clupeines have been examined as a funciton of pH, temperature, added salts, and presence of structure-disrupting agents
Moyra Lawrence et al.
Science advances, 8(7), eabj8618-eabj8618 (2022-02-17)
Platelet deficiency, known as thrombocytopenia, can cause hemorrhage and is treated with platelet transfusions. We developed a system for the production of platelet precursor cells, megakaryocytes, from pluripotent stem cells. These cultures can be maintained for >100 days, implying culture
L Ferrara et al.
European journal of biochemistry, 126(2), 389-394 (1982-08-01)
The three main fractions of clupeine, the protamine extracted from herring sperm, have been investigated by 13C nuclear magnetic resonance techniques. The dynamic behaviour, examined through the evaluation of the spin lattice relaxation times (T1) of individual carbon resonances assigned
N Navaratnam et al.
European journal of biochemistry, 171(3), 623-629 (1988-02-01)
Galactosyltransferase was purified from Golgi membranes of lactating-rat mammary gland and studied with respect to its physical and enzymic (lactose synthetase) properties. The enzyme occurred in both monomeric (43-46 kDa) and apparently dimeric (90 kDa) forms. It was very unstable
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