P0270

Calmodulin from bovine heart

lyophilized powder, 2,500-10,000 units/mg protein

• Synonyme(s) : Phosphodiesterase 3′:5′-cyclic nucleotide activator from bovine heart
• Numéro CAS: 77107-46-1
• Numéro MDL: MFCD00131869
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.61

Propriétés

• Source biologique: bovine heart
• Niveau de qualité: 200
• Forme: lyophilized powder
• Activité spécifique: 2,500-10,000 units/mg protein
• Composition: Protein, ≥85% Lowry
• Technique(s): calcium flux assay: suitable
• Numéro d'accès UniProt: P62157
• Température de stockage: −20°C
• Informations sur le gène: cow ... CALM3(520277)

Description

Application

This is the protein activator required for the Ca²⁺ dependent activation of phosphodiesterase, 3′:5′-cyclic nucleotide, P0520.

Actions biochimiques/physiologiques

Ca²⁺ binding protein that is required for activation of cyclic nucleotide-dependent phosphodiesterase. It is also a cofactor/activator of nitric oxide synthase, calcineurin, and many kinases including ATPase, myosin light chain kinase, and CAM kinase I, II, and III. It mediates ryanodine receptor activation by cyclic ADP ribose and is involved in intracellular Ca²⁺ homeostasis.

Définition de l'unité

One unit will stimulate 0.008 activated unit of phosphodiesterase, 3′:5′-cyclic nucleotide, P0520, in a 3 mL reaction volume at pH 7.5 and 30 °C, to 50% of the maximum activity of the enzyme when saturated with activator, in the presence of 0.01 mM Ca²⁺.

Forme physique

Lyophilized powder containing buffer salts as imidazole and magnesium sulfate

Notes préparatoires

Purified by a modification of the procedure of Teo, T.S., et al., J. Biol. Chem., 248, 588 (1973).

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)