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Key Documents

P0074

Sigma-Aldrich

Anti-PERK (C-terminal) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonyme(s) :

Anti-E2AK3, Anti-EIF2AK3, Anti-HsPEK, Anti-PEK, Anti-PRKR-like endoplasmic reticulum kinase, Anti-Pancreatic eIF2α kinase, Anti-WRS

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

Conjugué

unconjugated

Forme d'anticorps

IgG fraction of antiserum

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~150 kDa

Espèces réactives

human (predicted), mouse, rat

Technique(s)

western blot: 1:500-1:1,000 using whole cell lysate of HEK-293T expressing PERK cells

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

Description générale

Protein kinase RNA-like endoplasmic reticulum kinase (PERK) is one of the three mammalian unfolded protein response (UPR) transducers. It is a highly conserved eukaryotic initiation factor 2 α (eIF2α) kinase. The gene encoding this protein is localized on human chromosome 2p11.2.
Protein kinase RNA-like endoplasmic reticulum kinase (PERK) belongs to a family of eukaryotic initiation factor 2 α (eIF2α) kinases. It is a transmembrane kinase that is highly expressed in the pancreas. PERK contains two main domains: a kinase domain and an N-terminal domain like inositol- requiring enzyme-1 (IRE1), a protein involved in the unfolded protein response (UPR).

Spécificité

Anti-PERK C-terminal specifically recognizes PERK.

Immunogène

synthetic peptide corresponding to amino acids 1078-1092 of mouse PERK, conjugated to KLH. This sequence is identical in rat and differs by one amino acid in human.

Application

Anti-PERK (C-terminal) antibody produced in rabbit has been used for western blotting and ERK (extracellular-signal-regulated kinase) phosphorylation assays.

Actions biochimiques/physiologiques

Protein kinase RNA-like endoplasmic reticulum kinase (PERK) is an endoplasmic reticulum (ER) stress sensor which phosphorylates eukaryotic initiation factor 2 α (eIF2α), thereby reducing the translation of most messenger RNAs (mRNAs). In mice models of frontotemporal dementia, the suppression of translation in a PERK-eIF2α-dependent manner plays a key role in neuronal loss. PERK confers protection to heart against pressure overload-induced congestive heart failure. Mutations in this gene are linked with Wolcott-Rallison syndrome (WRS) which is characterized by permanent neonatal diabetes, exocrine-deficient pancreas, growth retardation and osteopenia.
Protein kinase RNA-like endoplasmic reticulum kinase (PERK) responds to distinct cellular stress signals.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Produit(s) apparenté(s)

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

The eIF2a kinase PERK limits the expression of hippocampal metabotropic glutamate receptor-dependent long-term depression.
Trinh MA
Learning & Memory (2014)
Role of the C-terminal di-leucine motif of 5-HT1A and 5-HT1B serotonin receptors in plasma membrane targeting
Damien Carrel
Journal of Cell Science (2006)
The structure of the PERK kinase domain suggests the mechanism for its activation
Cui W, et al.
Acta Crystallographica Section D, Biological Crystallography, 67(Pt 5), 423-423 (2011)
Perk Ablation Ameliorates Myelination in S63del-Charcot-Marie-Tooth 1B Neuropathy.
Musner N
ASN Neuro (2016)
PERK inhibition prevents tau-mediated neurodegeneration in a mouse model of frontotemporal dementia.
Radford H
Acta Neuropathologica (2015)

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