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P0072

Sigma-Aldrich

Anti-phospho-PDCD4 (pSer67) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonyme(s) :

Anti-Neoplastic transformation inhibitor, Anti-Nuclear antigen H731, Anti-Programmed cell death 4, Anti-Protein 197/15a

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~51 kDa

Espèces réactives

rat (predicted), mouse (predicted), human

Concentration

~1 mg/mL

Technique(s)

immunoprecipitation (IP): 5-10 μg using lysates of HEK-293T cells starved for 48 hours and then treated with 20% FCS
western blot: 1-2 μg/mL using lystes of HEK-293T cells starved for 48 hours and then treated with 20% FCS

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

phosphorylation (pSer67)

Informations sur le gène

Description générale

Programmed cell death 4 (PDCD4) also known as nuclear antigen H731-like, Protein 197/15a, neoplastic transformation inhibitor protein), is a tumor suppressor protein that is lost in progressed carcinomas of lung, breast, colon and prostate.

Application

Anti-phospho-PDCD4 (pSer67) antibody produced in rabbit has been used in immunoblotting.

Actions biochimiques/physiologiques

Programmed cell death 4 (PDCD4) suppresses translation initiation by specifically inhibiting the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex and by competing with eIF4G, a second component of the translation initiation complex, for binding to eIF4A. In response to mitogens, PDCD4 was rapidly phosphorylated on Ser67 by the protein kinase S6K1 and subsequently degraded via the ubiquitin ligase SCF-β (TRCP). It has been shown that the protein is specifically phosphorylated on Ser67 and Ser457 by Akt, both in vitro and in vivo. This phosphorylation causes nuclear translocation of PDCD4.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

S6K1- and betaTRCP-mediated degradation of PDCD4 promotes protein translation and cell growth
Dorrello NV, et al.
Science (New York, N.Y.), 314(5798), 467-471 (2006)
Programmed Cell Death 4 (PDCD4): A Novel Player in Ethanol Mediated Suppression of Protein Translation in Primary Cortical Neurons and Developing Cerebral Cortex
Narasimhan M, et al.
Alcoholism, Clinical and Experimental Research, 37(1), 96-96 (2013)
Programmed cell death protein 4 suppresses CDK1/cdc2 via induction of p21Waf1/Cip1
Goke R, et al.
American Journal of Physiology. Cell Physiology, C1541-C1546 (2004)
Aleksandr Barulin et al.
Nano letters, 19(10), 7434-7442 (2019-09-19)
Single molecule detection provides detailed information about molecular structures and functions but it generally requires the presence of a fluorescent marker which can interfere with the activity of the target molecule or complicate the sample production. Detecting a single protein
Programmed cell death protein 4 suppresses CDK1/cdc2 via induction of p21Waf1/Cip1
Goke R, et al.
American Journal of Physiology. Cell Physiology, 287(6), C1541-C1546 (2004)

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