N0513
Nerve Growth Factor-7S from murine submaxillary gland
lyophilized powder, suitable for cell culture
Synonyme(s) :
NGF-7S
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About This Item
Numéro CAS:
Numéro CE :
Numéro MDL:
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.77
Produits recommandés
Nom du produit
Nerve Growth Factor-7S from murine submaxillary gland, NGF-7S, lyophilized powder, suitable for cell culture
Source biologique
mouse
Niveau de qualité
Forme
lyophilized powder
Puissance
2-250 ng/mL ED50/EC50
Qualité
endotoxin tested
Poids mol.
protein 130 kDa
Conditionnement
pkg of 0.1 mg
Technique(s)
cell culture | mammalian: suitable
Impuretés
≤10
Couleur
white
Numéro d'accès UniProt
Température de stockage
−20°C
Informations sur le gène
mouse ... Ngf(18049)
Description générale
Nerve growth factor (NGF) is a dimer of 118-residue polypeptide chains linked non-covalently. Both these polypeptides contain three intramolecular disulphide bridges. It forms the prototype of the neurotrophin growth factor family. In male mouse submandibular salivary gland, NGF is present in high amounts, and is present as a high molecular weight complex called nerve growth factor-7S (NGF-7S). NGF-7S is an α2β2γ2 complex, where β2-NGF is the active neurotrophin, and is linked to the dimers of the glandular kallikrein serine proteinases members, α-NGF and γ-NGF.
Application
Nerve Growth Factor-7S (NGF-7S) from murine submaxillary gland has been used for the treatment of following cells to induce neuronal differentiation-
- PC12
- Dorsal root ganglia (DRG) obtained from chick embryos of either sex (Gallus gallus)
Actions biochimiques/physiologiques
Nerve growth factor (NGF) is a neurotrophin, which facilitates the differentiation and survival of specific subtypes of neurons of central nervous system and peripheral nervous system. However, nerve growth factor-7S (NGF-7S) lacks any neurotrophic activity as most part of β-NGF subunit is buried within the NGF-7S complex.
Forme physique
Lyophilized from 0.2 μm filtered solution in 25 mM sodium phosphate, pH 7.0.
Notes préparatoires
Nerve growth factor-7S (NGF-7S) is isolated from male mouse submaxillary glands using a modification of the method of Varon, et al.
Remarque sur l'analyse
The biological activity is measured using a 3-day MTT assay.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
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