Accéder au contenu
MilliporeSigma
Toutes les photos(10)

Key Documents

MTOX1030

Sigma-Aldrich

Kidney PTEC Control Cell (SA7K Clone)

Synonyme(s) :

RPTEC SA7K control cell line

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme


About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.24

Source biologique

human female renal (proximal convoluted tubule epithelial cells)

Niveau de qualité

Description

1 vial

Forme

liquid

Température de stockage

−196°C

Description générale

Sigma modified human primary renal proximal tubule epithelial cells (RPTEC) with ZFNs to effectively create novel human RPTEC cell lines that can be utilized for investigating kidney toxicology (nephrotoxicity), transporters and various other renal studies. Sigma′s RPTEC′s exhibit normal epithelial morphology and expression of Renal Proximal Tubule (PT) specific markers such as, CD13 and α-GST. Sigma′s RPTEC′s maintain typical PT functions including albumin reabsorption, γ-glutamyl transpeptidase (GGT) activity, and respond to parathyroid hormone. The cells respond to several known human nephrotoxicants similar to human primary cells and demonstrate robust expression and activity of key kidney uptake and efflux transporters incuding OCT2, OATP4C1, OAT1, OCTN1, MRP2, MRP4, P-gp, MATE1, MATE2-K, PEPT1, and PEPT2.

Application

Human RPTEC SA7K control cell line and transporter KO models have been validated in several standard and 3D assays for measuring nephrotoxicity and transporter assays. RPTEC SA7K control cells can be used to measure toxicity of compounds using dose-response curves and viability such as MTT based in vitro Toxicology in both 2D and 3D culture.

RPTEC SA7K control cells can also be used to measure compound uptake/efflux by transporters using inhibitors. Precision editing in RPTEC SA7K transporter KO cells provides a more predictive platform for compound uptake/efflux by transporters without the need for non-specific inhibitors.

RPTEC SA7K cells have been validated for use on the Mimetas 3-lane OrganoPlate® in both toxicity and barrier integrity assays ( Z742750).


RPTEC SA7K cells can also be used on the AIM Biotech 3D cell culture chip in toxicity and permeability assays ( DAX01).

Caractéristiques et avantages

Sigma′s human RPTEC control SA7K clone cells exhibit normal renal proximal tubule characteristics, functional renal uptake and efflux transporters and are sensitive to human nephrotoxicants making the SA7K clone ideal for studying drug-transporter interactions and early detection of renal toxicants. The SA7K clone should be used as a control for Sigma′s human RPTEC knockout (KO) cells.

Qualité

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation

Informations légales

These products are covered by the ADME/Tox Cell Lines License as described in Exhibit 1, in the technical bulletin.
OrganoPlate is a registered trademark of MIMETAS B.V.

Clause de non-responsabilité

Ce produit, destiné à la recherche scientifique, est soumis à une réglementation spécifique en France, y compris pour les activités d'importation et d'exportation (Article L 1211-1 alinéa 2 du Code de la Santé Publique). L'acheteur (c'est-à-dire l'utilisateur final) est tenu d'obtenir une autorisation d'importation auprès du Ministère français de la Recherche, mentionné à l'article L1245-5-1 II du Code de la Santé Publique. En commandant ce produit, vous confirmez détenir l'autorisation d'importation requise.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Marianne K Vormann et al.
The AAPS journal, 20(5), 90-90 (2018-08-16)
Proximal tubules in the kidney play a crucial role in reabsorbing and eliminating substrates from the body into the urine, leading to high local concentrations of xenobiotics. This makes the proximal tubule a major target for drug toxicity that needs

Articles

AIM Biotech’s easy-to-use modular platform for conducting 3D cell culture into your research. The AIM Biotech organ-on-a-chip enables you to run simple assays or create more complex microphysiological systems. The versatile platform is suitable for a wide variety of cell types and is customizable for your specific needs.

Human renal proximal tubule epithelial cells (RPTECs) are commonly used to predict human renal drug permeability and to investigate drug efflux. We have generated transporter knockout (KO) cell lines using CompoZr® Zinc Finger Nuclease (ZFN) technology in a proprietary renal proximal tubule epithelial cell line.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique