MTOX1011

PXR Knockout HepaRG^™ Cells

human female liver (Source Disease: Hepatocarcinoma and Hepatitis C)

• Code UNSPSC : 12352207
• Nomenclature NACRES : NA.81

Propriétés

• product name: PXR Knockout HepaRG^™ Cells, 1 vial
• Source biologique: human female liver (Source Disease: Hepatocarcinoma and Hepatitis C)
• Forme: liquid
• Numéro d'accès OMIM: 603065
• Température de stockage: −196°C
• Informations sur le gène: human ... NR1I2(1337040)

Description

Description générale

HepaRG is a human hepatoma cell line. The cells possess a pseudodiploid karyotype and have been characterized as an oval ductular bipotent hepatic cell line as they have the ability to differentiate into both biliary and hepatocyte lineages in the presence of DMSO. HepaRG PXR KO cells express the major xenobiotic sensors (CAR and AhR), drug transporters, phase I and II drug metabolizing enzymes as well as key hepatic transcription factors involved in stress response pathways.

Application

See technical bulletin for detailed protocols

Caractéristiques et avantages

HepaRG cells are the most metabolically active human hepatocyte cell line developed to date. These cells are suitable for a wide variety of studies for drug metabolism, CYP induction, metabolism-mediated toxicity, transporter, and hepatotoxicity.

• Sigma′s HepaRG PXR Knockout (KO) allows conclusive identification of CYP3A4 induction through PXR activation.

Zinc finger nucleases (ZFN) mediated Knock-out of NR1I2 (PXR) gene.

• The frame-shift mutation of NR1I2 gene was confirmed by fragment length analysis and DNA sequencing.
• Loss of functionality was confirmed by loss of response to inducers of cytochrome P450 activity.

Qualité

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation

Informations légales

These products are covered by the License Agreement as described in Exhibit 1 and 2, in the technical bulletin.

HepaRG is a trademark of BioPredic International company

Clause de non-responsabilité

Ce produit, destiné à la recherche scientifique, est soumis à une réglementation spécifique en France, y compris pour les activités d'importation et d'exportation (Article L 1211-1 alinéa 2 du Code de la Santé Publique). L'acheteur (c'est-à-dire l'utilisateur final) est tenu d'obtenir une autorisation d'importation auprès du Ministère français de la Recherche, mentionné à l'article L1245-5-1 II du Code de la Santé Publique. En commandant ce produit, vous confirmez détenir l'autorisation d'importation requise.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable